• Login
    View Item 
    •   NM-AIST Home
    • Life sciences and Bio-engineering
    • Research Articles [LISBE]
    • View Item
    •   NM-AIST Home
    • Life sciences and Bio-engineering
    • Research Articles [LISBE]
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Potential Opportunities and Challenges of Deploying Next Generation Sequencing and CRISPR-Cas Systems to Support Diagnostics and Surveillance Towards Malaria Control and Elimination in Africa

    Thumbnail
    View/Open
    Full text (2.992Mb)
    Date
    2022-07-13
    Author
    Lyimo, Beatus
    Popkin-Hall, Zachary
    Giesbrecht, David
    Mandara, Celine
    Madebe, Rashid
    Bakari, Catherine
    Pereus, Dativa
    Seth, Misago
    Ngamba, Ramadhan
    Mbwambo, Ruth
    MacInnis, Bronwyn
    Mbwambo, Daniel
    Garimo, Issa
    Chacky, Frank
    Aaron, Sijenunu
    Lusasi, Abdallah
    Molteni, Fabrizio
    Njau, Ritha
    Cunningham, Jane
    Lazaro, Samwel
    Mohamed, Ally
    Juliano, Jonathan
    Bailey, Jeffrey
    Ishengoma, Deus
    Metadata
    Show full item record
    Abstract
    Recent developments in molecular biology and genomics have revolutionized biology and medicine mainly in the developed world. The application of next generation sequencing (NGS) and CRISPR-Cas tools is now poised to support endemic countries in the detection, monitoring and control of endemic diseases and future epidemics, as well as with emerging and re-emerging pathogens. Most low and middle income countries (LMICs) with the highest burden of infectious diseases still largely lack the capacity to generate and perform bioinformatic analysis of genomic data. These countries have also not deployed tools based on CRISPR-Cas technologies. For LMICs including Tanzania, it is critical to focus not only on the process of generation and analysis of data generated using such tools, but also on the utilization of the findings for policy and decision making. Here we discuss the promise and challenges of NGS and CRISPR-Cas in the context of malaria as Africa moves towards malaria elimination. These innovative tools are urgently needed to strengthen the current diagnostic and surveillance systems. We discuss ongoing efforts to deploy these tools for malaria detection and molecular surveillance highlighting potential opportunities presented by these innovative technologies as well as challenges in adopting them. Their deployment will also offer an opportunity to broadly build in-country capacity in pathogen genomics and bioinformatics, and to effectively engage with multiple stakeholders as well as policy makers, overcoming current workforce and infrastructure challenges. Overall, these ongoing initiatives will build the malaria molecular surveillance capacity of African researchers and their institutions, and allow them to generate genomics data and perform bioinformatics analysis in-country in order to provide critical information that will be used for real-time policy and decision-making to support malaria elimination on the continent.
    URI
    https://doi.org/10.3389/fcimb.2022.757844
    https://dspace.nm-aist.ac.tz/handle/20.500.12479/2349
    Collections
    • Research Articles [LISBE]

    Nelson Mandela-AIST copyright © 2021  DuraSpace
    Theme by 
    Atmire NV
     

     

    Browse

    All PublicationsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Nelson Mandela-AIST copyright © 2021  DuraSpace
    Theme by 
    Atmire NV