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dc.contributor.authorNkabinde, L. A.
dc.contributor.authorShoba-Zikhali, L. N. N.
dc.contributor.authorSemete-Makokotlela, B.
dc.contributor.authorKalombo, L.
dc.contributor.authorSwai, Hulda
dc.contributor.authorGrobler, A.
dc.contributor.authorHamman, J. H.
dc.date.accessioned2020-08-31T07:29:39Z
dc.date.available2020-08-31T07:29:39Z
dc.date.issued2014
dc.identifier.urihttps://doi.org/10.3144/expresspolymlett.2014.23
dc.identifier.urihttps://dspace.nm-aist.ac.tz/handle/20.500.12479/890
dc.descriptionThis research article published by eXPRESS Polymer Letters Vol.8, No.3 (2014)en_US
dc.description.abstractNanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within the lysosomal compartment and their cytotoxicity in different cell lines. Rhodamine-6G labelled poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation freeze-drying method. Uptake and co-localisation of PLGA nanoparticles in lysosomes were visualized by confocal laser scanning microscopy. The cytotoxicity of the nanoparticles was evaluated on different mammalian cells lines by means of Trypan blue exclusion and the MTS assay. The PLGA nanoparticles accumulated in the intercellular spaces of Caco-2 cell monolayers, but were also taken up transcellularly into the Caco-2 cells and partially co-localized within the lysosomal compartment indicating involvement of endocytosis during uptake. PLGA nanoparticles did not show cytotoxic effects in all three cell lines. Intact PLGA nanoparticles are therefore capable of moving across epithelial cell membranes partly by means of endocytosis without causing cytotoxic effects.en_US
dc.language.isoenen_US
dc.publishereXPRESS Polymer Lettersen_US
dc.subjectBiocompatible polymersen_US
dc.subjectCaco-2 cellsen_US
dc.subjectCellular uptakeen_US
dc.subjectPLGA nanoparticlesen_US
dc.titlePoly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicityen_US
dc.typeArticleen_US


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