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dc.contributor.authorAkoko, James
dc.contributor.authorPelle, Roger
dc.contributor.authorKivali, Velma
dc.contributor.authorSchelling, Esther
dc.contributor.authorMachuka, Eunice
dc.contributor.authorMathew, Coletha
dc.contributor.authorFèvre, Eric
dc.contributor.authorKyallo, Victoria
dc.contributor.authorFalzon, Laura
dc.contributor.authorLukambagire, Abdul
dc.contributor.authorHalliday, Jo
dc.contributor.authorBonfoh, Bassirou
dc.contributor.authorKazwala, Rudovick
dc.contributor.authorOuma, Collins
dc.contributor.authorShirima, Gabriel
dc.date.accessioned2020-06-12T08:38:17Z
dc.date.available2020-06-12T08:38:17Z
dc.date.issued2020-05-11
dc.identifier.urihttps://doi.org/10.1186/s12917-020-02346-y
dc.identifier.urihttps://dspace.nm-aist.ac.tz/handle/20.500.12479/792
dc.descriptionThis research article published by BioMed Central Ltd, 2020en_US
dc.description.abstractBackground: Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results: A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RTPCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion: The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.en_US
dc.language.isoenen_US
dc.publisherBioMed Central Ltden_US
dc.subjectSerologyen_US
dc.subject“Molecular detection”en_US
dc.subject“Molecular evidence”en_US
dc.subject“Pig brucellosis”en_US
dc.titleSerological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya.en_US
dc.typeArticleen_US


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