Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya.

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dc.contributor.author Akoko, James
dc.contributor.author Pelle, Roger
dc.contributor.author Kivali, Velma
dc.contributor.author Schelling, Esther
dc.contributor.author Machuka, Eunice M
dc.contributor.author Mathew, Coletha
dc.contributor.author Fèvre, Eric M
dc.contributor.author Kyallo, Victoria
dc.contributor.author Falzon, Laura C
dc.contributor.author Lukambagire, AbdulHamid S
dc.contributor.author Halliday, Jo E. B.
dc.contributor.author Bonfoh, Bassirou
dc.contributor.author Kazwala, Rudovick
dc.contributor.author Ouma, Collins
dc.contributor.author Shirima, Gabriel M.
dc.date.accessioned 2020-06-12T08:38:17Z
dc.date.available 2020-06-12T08:38:17Z
dc.date.issued 2020-05-11
dc.identifier.uri https://doi.org/10.1186/s12917-020-02346-y
dc.identifier.uri https://dspace.nm-aist.ac.tz/handle/20.500.12479/792
dc.description This research article published by BioMed Central Ltd, 2020 en_US
dc.description.abstract Background: Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results: A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RTPCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion: The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs. en_US
dc.language.iso en en_US
dc.publisher BioMed Central Ltd en_US
dc.subject Serology en_US
dc.subject “Molecular detection” en_US
dc.subject “Molecular evidence” en_US
dc.subject “Pig brucellosis” en_US
dc.title Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya. en_US
dc.type Article en_US

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