| dc.description.abstract | This research investigated the effects of sciatic nerve transection and diabetes on the hippocampus, and
the protective effects of
Garcinia kola and curcumin. Thirty-five adult male Wistar albino rats were divided
into five groups: a control group (Cont), a transected group (Sham group), a transected + diabetes mellitus
group (DM), a transected + diabetes mellitus +
Garcinia kola group (DM + GK), and a transected + DM +
curcumin group (DM + Cur), each containing seven animals. The experimental diabetes model was
created with the intraperitoneal injection of a single dose of streptozotocin. No procedure was applied to
the Cont group, while sciatic nerve transection was performed on the other groups.
Garcinia kola was
administered to the rats in DM + GK, and curcumin to those in DM + Cur. Cardiac perfusion was performed
at the end of the experimental period. Brain tissues were dissected for stereological, histopathological,
and immunohistochemical evaluations. The volume ratios of hippocampal layers to the entire
hippocampus volume were compared between the groups. Anti-S100, anti-caspase 3, and anti-SOX 2
antibodies were used for immunohistochemical analysis. No statistically significant difference was
observed in the volume ratios of the four hippocampal layers. However, the volume ratio of the stratum
lucidum was higher in the Sham, DM, and DM + Cur groups compared to the Cont group. While curcumin
exhibited a protective effect on hippocampal tissue following diabetes induction,
Garcinia kola had only a
weak protective effect. Increased cell density and nuclear deterioration due to diabetes and nerve
transection can be partially ameliorated by treatment with
Garcinia kola and curcumin | en_US |
