| dc.description.abstract | The transmission of signals to the cell body from injured axons induces significant alterations in primary sensory
neurons located in the ganglion tissue, the site of the perikaryon of the affected nerve fibers. Disruption of the
continuity between the proximal and distal ends leads to substantial adaptability in ganglion cells and induces
macrophage-like activity in the satellite cells. Research findings have demonstrated the plasticity of satellite cells
following injury. Satellite cells work together with sensory neurons to extend the interconnected surface area in
order to permit effective communication. The dynamic cellular environment within the ganglion undergoes
several alterations that ultimately lead to differentiation, transformation, or cell death. In addition to necrotic
and apoptotic cell morphology, phenomena such as histomorphometric alterations, including the development of
autophagic vacuoles, chromatolysis, cytosolic degeneration, and other changes, are frequently observed in cells
following injury. The use of electron microscopic and stereological techniques for assessing ganglia and nerve
fibers is considered a gold standard in terms of investigating neuropathic pain models, regenerative therapies,
some treatment methods, and quantifying the outcomes of pharmacological and bioengineering interventions.
Stereological techniques provide observer-independent and reliable results, which are particularly useful in the
quantitative assessment of three-dimensional structures from two-dimensional images. Employing the fraction ator and disector techniques within stereological methodologies yields unbiased data when assessing parameters
such as number. The fundamental concept underlying these methodologies involves ensuring that each part of
the structure under evaluation has an equal opportunity of being sampled. This review describes the stereological
and histomorphometric evaluation of dorsal root ganglion neurons and satellite cells following nerve injury
models. | en_US |
