Browsing by Author "Kaplan, Suleyman"

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Beneficial effects of curcumin in the diabetic rat ovary: a stereological and biochemical study
(Research Square, 2022-08-25) Tufekc, Kıymet; Kaplan, Suleyman
Study investigated the effects of curcumin treatment on diabetic ovaries at different periods of the disease. Fifty-six female Wistar albino rats (250–300 g) aged 12 weeks were divided into seven groups. No treatment was applied to the control group. The sham group was given 5 mL/kg of corn oil, and the curcumin group 30 mg/kg curcumin. In the DM groups, diabetes was induced by a single intraperitoneal dose of 50 mg/kg streptozotocin (STZ). The DM-treated groups received 30 mg/kg curcumin after either seven days (DC1 group), or 21 days (DC2 group), or simultaneously with STZ injection (DC3 group). Numbers of follicles in the ovaries were estimated using stereological method. FSH, LH, and SOD levels and CAT activity were measured in serum specimens. Follicle numbers and volumes of corpus luteum, blood vessel and cortex volumes, gonadosomatic index, and FSH and SOD levels all decreased signicantly in diabetic ovaries, while relative weight loss, connective tissue volume, and CAT activity increased (p < 0.01). Curcumin treatment had a protective effect on the number of primordial follicles in the DC2 group and on antral follicle numbers in the DC3 group. Curcumin also exhibited positive effects on CAT activity and SOD levels, blood glucose levels, and corpus luteum, connective tissue and blood vessel volumes in the DC2 and DC3 groups. Curcumin also ameliorated FSH levels in the DC1 and DC3 groups (p < 0.01). Curcumin exhibits protective effects on ovarian structures and folliculogenesis, especially when used concurrently with the development of diabetes or in later stages of the disease
The effect of dietary sesame oil and ginger oil as antioxidants in the adult rat dorsal root ganglia after peripheral nerve crush injury
(Taylor & Francis online, 2022-11-20) Delibaş, Burcu; Kaplan, Arife; Marangoz, Abdullah; Eltahir, Mohammed; Altun, Gamze; Kaplan, Suleyman
Aim: The purpose of this study was to investigate the effect of dietary sesame oil and ginger oil supplements on the dorsal root ganglia following a sciatic nerve crush model in male Wistar albino rats. Materials and methods: Crush injury models have been done by means of graded forceps (50 Newton). The animals were given a daily sesame oil (4ml/kg/day) and ginger oil (400mg/kg/ day) via oral gavage for a period of 28days. Dorsal root ganglia from the L5 levels were harvested. Processing of tissues was done for electron microscopy and light microscopy. Immunohistochemical staining with active caspase-3 antibody and qualitative ultrastructural analyses of tissues were made by a light and a transmission electron microscope, respectively. Results: The results showed that crush injury leads to remarkable ultrastructural changes in sensory neurons, such as swollen mitochondria, disruption of cristae structure, glial cell proliferation and, consequently, phagocytosis of the damaged neuron. These ultrastructural changes were less evident in the treated groups, and both natural compounds reduced the expression of activated caspase-3, which may also affect ultrastructural changes. Conclusion: The application of the natural products sesame oil and ginger oil may represent a supportive approach to the protection of sensory neurons against the destructive effects of peripheral nerve crush injury
The histomorphological and stereological assessment of rat dorsal root ganglion tissues after various types of sciatic nerve injury
(Research Square, 2023-08-01) Delibaş, Burcu; Kaplan, Suleyman
Peripheral nerve injuries lead to significant changes in the dorsal root ganglia, where the cell bodies of the damaged axons are located. The sensory neurons and the surrounding satellite cells rearrange the composition of the intracellular organelles to enhance their plasticity for adaptation to changing conditions and responding to injury. Meanwhile, satellite cells acquire phagocytic properties and work with macrophages to eliminate degenerated neurons. These structural and functional changes are not identical in all injury types. Understanding the cellular response, which varies according to the type of injury involved, is essential in determining the optimal method of treatment. In this research, we investigated the numerical and morphological changes in primary sensory neurons and satellite cells in the dorsal root ganglion 30 days following chronic compression, crush and transection injuries using stereology, high-resolution light microscopy, immunohistochemistry, and behavioral analysis techniques. Electron microscopic methods were employed to evaluate ne structural alterations in cells. Stereological evaluations revealed no statistically signicant difference in terms of mean sensory neuron numbers (p > 0.05), although a signicant decrease was observed in sensory neuron volumes in the transection and crush injury groups (p < 0.05). Active caspase-3 immunopositivity increased in the injury groups compared to the sham group (p < 0.05). While crush injury led to desensitization, chronic compression injury caused thermal hyperalgesia. Electromyography parameters exhibited a significant decrease in the compression and crush injury groups compared to the sham group (p < 0.05). Macrophage infiltration were observed in all injury types. Electron microscopic results revealed that the chromatolysis response was triggered in the sensory neuron bodies from the transection injury group. An increase in organelle density was observed in the perikaryon of sensory neurons after crush-type injury. This indicates the presence of a more active regeneration process in crush-type injury than in other types. The effect of chronic compression injury is more devastating than that of crush-type injury, and the edema caused by compression significantly inhibits the regeneration process.
The neuroprotective effects of baobab and black seed on the rat hippocampus exposed to a 900-MHz electromagnetic field
(Elsevier, 2024-04) Mohamed, Hamza; Deniz, Omur; Kaplan, Suleyman
This study investigated the potential effects on the hippocampus of electromagnetic fields (EMFs) disseminated by mobile phones and the roles of baobab (Adansonia digitata) (AD) and black seed (Nigella sativa) (BS) in mitigating these. Fifty-six male, 12-week-old Wistar albino rats were divided into eight groups of seven animals each. No EMF exposure was applied to the control, AD or BS groups, while the rats in the Sham group were placed in an EMF system with no exposure. A 900-MHz EMF was applied to the EMF+AD, EMF+BS, EMF+AD+BS and EMF groups for 1 hour a day for 28 days. Pyramidal neurons in the hippocampus were subsequently counted using the optical fractionator technique, one of the unbiased stereological methods. Tissue sections were also evaluated histopathologically under light and electron microscopy. The activities of the enzymes catalase (CAT) and superoxide dismutase (SOD) were also determined in blood serum samples. Analysis of the stereological data revealed no statistically significant differences between the EMF and control or sham groups in terms of pyramidal neuron numbers (p>0.05). However, stereological examination revealed a crucial difference in the entire hippocampus between the control group and the AD (p<0.01) and BS (p<0.05) groups. Moreover, exposure to 900-MHz EMF produced adverse changes in the structures of neurons at histopathological analysis. Qualitative examinations suggest that a combination of herbal products such as AD and BS exerts a protective effect against such EMF side-effects.
Regeneration responses of Ankaferd Blood Stopper, platelet-rich-plasma, and Momordica charantia on sciatic nerve injury in the obese rats
(IOP Publishing, 2025-05-23) Önger, Mehmet; Geuna, Stefano; El Elhaj, Abubaker; Raimondo, Stefania; Kaplan, Suleyman
Obesity is a prevalent and potentially fatal disorder in industrialized nations, often due to an imbalance between calorie intake and energy expenditure. The study investigates the effects of Ankaferd Blood Stopper (ABS), platelet-rich plasma (PRP), and Momordica charantia (MC) on obese rats with sciatic nerve injuries using stereological and electron microscopic techniques. Twenty-four female Sprague Dawley rats, aged 8–10 weeks, were divided into three groups: the obese gap ABS group (OGABS), the obese gap PRP group (OGPRP), and the obese gap MC group (OGMC). Afive-mm nerve block was resected approximately 10 mm above the nerve branch. The gap region was then surrounded and closed by a collagen membrane in tube form. Materials such as ABS, PRP, and MCwere injected into the tubes of each group. Electromyography and histological procedures were performed after 12 weeks of surgery to investigate their structural repair and functional promotion of nerve regeneration. A significant increase in the number of myelinated axons in OGMCwasfound.Inaddition, the latency values in OGMC were more critical than in the OGABSandOGPRPgroups,indicating better myelination. PRP and ABS positively affected nerve gap repair, favoring regenerating unmyelinated axons. Due to increased amplitude values, PRP and ABS have also improved axonal area regeneration. Collagen tubes containing ABS, PRP, and MC may help repair and close peripheral nerve gaps in obese rats, which could be beneficial in closing peripheral nerve resection and demyelination. The study’s findings indicate that these collagen tubes can have a therapeutic effect on peripheral nerve regeneration in obese models.