Browsing by Author "El Elhaj, Abubaker"

Now showing 1 - 2 of 2

Regeneration responses of Ankaferd Blood Stopper, platelet-rich-plasma, and Momordica charantia on sciatic nerve injury in the obese rats
(IOP Publishing, 2025-05-23) Önger, Mehmet; Geuna, Stefano; El Elhaj, Abubaker; Raimondo, Stefania; Kaplan, Suleyman
Obesity is a prevalent and potentially fatal disorder in industrialized nations, often due to an imbalance between calorie intake and energy expenditure. The study investigates the effects of Ankaferd Blood Stopper (ABS), platelet-rich plasma (PRP), and Momordica charantia (MC) on obese rats with sciatic nerve injuries using stereological and electron microscopic techniques. Twenty-four female Sprague Dawley rats, aged 8–10 weeks, were divided into three groups: the obese gap ABS group (OGABS), the obese gap PRP group (OGPRP), and the obese gap MC group (OGMC). Afive-mm nerve block was resected approximately 10 mm above the nerve branch. The gap region was then surrounded and closed by a collagen membrane in tube form. Materials such as ABS, PRP, and MCwere injected into the tubes of each group. Electromyography and histological procedures were performed after 12 weeks of surgery to investigate their structural repair and functional promotion of nerve regeneration. A significant increase in the number of myelinated axons in OGMCwasfound.Inaddition, the latency values in OGMC were more critical than in the OGABSandOGPRPgroups,indicating better myelination. PRP and ABS positively affected nerve gap repair, favoring regenerating unmyelinated axons. Due to increased amplitude values, PRP and ABS have also improved axonal area regeneration. Collagen tubes containing ABS, PRP, and MC may help repair and close peripheral nerve gaps in obese rats, which could be beneficial in closing peripheral nerve resection and demyelination. The study’s findings indicate that these collagen tubes can have a therapeutic effect on peripheral nerve regeneration in obese models.
The effects of benzimidazole and electrical stimulation on peripheral nerve regeneration after short‑ and long‑term injury
(Histochemistry and Cell Biology, 2025-05) El Elhaj, Abubaker; Elamin, Abdalla; Kaplan, Süleyman
This research investigated the effects of benzimidazole (BZ) and electrical stimulation (ES) on peripheral nerve regeneration after short- and long-term injury and assessed functional recovery by means of stereological, histological, and electrophysiological analyses. Fifty-four male albino Wistar rats were divided into nine groups of six animals each. No treatment or surgery was applied to the control (CONT) group. The sciatic nerve was crushed for 5 s in the short-term injury (STI) and for 60 s in the long-term injury (LTI) groups. In the STI + BZ group and the LTI + BZ group, the rats received 25 mg/kg/day of BZ via oral gavage for 28 days. In the STI + ES and LTI + ES groups, a 3-V current was applied for 20 min daily for 28 days. In the STI + BZ + ES group and the LTI + BZ + ES groups, 3-V ES was applied for 20 min per day for 28 days following oral administration of BZ at 25 mg/kg/day for 28 days. All groups were subjected to electrophysiological, electron microscopic, stereological, and statistical analyses. The stereological analyses revealed a significant increases in the numbers of myelinated axons in the STI + ES groups compared with the STI (p < 0.01). BZ treatment yielded no significant differences in the numbers of myelinated axons in the groups (p > 0.05). Histological evaluation of the STI and LTI groups showed that ES and BZ treatment positively affect the histological structure of the nerve.