Performance of Tuberculosis Molecular Bacterial Load Assay Compared to Alere TB-LAM in Urine of Pulmonary Tuberculosis Patients with HIV Co-Infections
| dc.contributor.author | Mapamba, Daniel | |
| dc.contributor.author | Sauli, Elingarami | |
| dc.contributor.author | Lalashowi, Julieth | |
| dc.contributor.author | Buza, Joram | |
| dc.contributor.author | John, Joseph | |
| dc.contributor.author | Mwaisango, Zawadi | |
| dc.contributor.author | Tarmo, Peter | |
| dc.contributor.author | Sabi, Issa | |
| dc.contributor.author | Rachow, Andrea | |
| dc.contributor.author | Ntinginya, Nyanda | |
| dc.contributor.author | Mtafya, Bariki | |
| dc.date.accessioned | 2023-04-28T10:51:16Z | |
| dc.date.available | 2023-04-28T10:51:16Z | |
| dc.date.issued | 2023-02-23 | |
| dc.description | This research article was published by MDPI in 2023 | en_US |
| dc.description.abstract | Alternative tools are needed to improve the detection of M. tuberculosis (M. tb) in HIV co-infections. We evaluated the utility of Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) compared to lipoarabinomannan (LAM) to detect M. tb in urine. Sputum Xpert MTB/RIF-positive patients were consented to provide urine at baseline, weeks 2, 8, 16, and 24 of treatment for TB-MBLA, culture, and LAM. Results were compared with sputum cultures and microscopy. Initial M. tb. H37Rv spiking experiments were performed to validate the tests. A total of 63 urine samples from 47 patients were analyzed. The median age (IQR) was 38 (30–41) years; 25 (53.2%) were male, 3 (6.5%) had urine for all visits, 45 (95.7%) were HIV positive, of whom 18 (40%) had CD4 cell counts below 200 cells/µL, and 33 (73.3%) were on ART at enrollment. Overall urine LAM positivity was 14.3% compared to 4.8% with TB-MBLA. Culture and microscopy of their sputum counterparts were positive in 20.6% and 12.7% of patients, respectively. Of the three patients with urine and sputum at baseline, one (33.33%) had urine TB-MBLA and LAM positive compared to 100% with sputum MGIT culture positive. Spearman’s rank correction coefficient (r) between TB-MBLA and MGIT was −0.85 and 0.89 with a solid culture, p > 0.05. TB-MBLA has the promising potential to improve M. tb detection in urine of HIV-co-infected patients and complement current TB diagnostics. | en_US |
| dc.identifier.uri | https://doi.org/10.3390/ijms24043715 | |
| dc.identifier.uri | https://dspace.nm-aist.ac.tz/handle/20.500.12479/1888 | |
| dc.language.iso | en | en_US |
| dc.publisher | MDPI | en_US |
| dc.subject | TB-lipoarabinomannan | en_US |
| dc.subject | Tuberculosis-molecular bacterial load assay | en_US |
| dc.subject | Human immunodeficiency virus | en_US |
| dc.title | Performance of Tuberculosis Molecular Bacterial Load Assay Compared to Alere TB-LAM in Urine of Pulmonary Tuberculosis Patients with HIV Co-Infections | en_US |
| dc.type | Article | en_US |