DENV-2 Circulation and Host Preference Among Highly Anthropophilic, Outdoor-Biting Aedes aegypti in Dar es Salaam, Tanzania

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dc.contributor.authorTenywa, Frank
dc.contributor.authorHälg, Silvan
dc.contributor.authorMakame, Haji
dc.contributor.authorMoore, Jason
dc.contributor.authorDogan, Osward
dc.contributor.authorMapipi, Harubu
dc.contributor.authorMachange, Jane
dc.contributor.authorLilolime, Nasoro
dc.contributor.authorHofer, Lorenz
dc.contributor.authorBatao, Lewis
dc.contributor.authorMwamlima, Tunu
dc.contributor.authorMüller, Pie
dc.contributor.authorMoore, Sarah
dc.date.accessioned2025-09-20T13:37:57Z
dc.date.issued2025-06-05
dc.descriptionSDG - 3: Good Health and Well-being SDG - 6: Clean Water and Sanitation SDG - 11: Sustainable Cities and Communities SDG - 13: Climate Action SDG - 17: Partnerships for the Goals
dc.description.abstractIn Tanzania, dengue outbreaks have occurred almost annually over the past decade, with each new outbreak becoming more severe. This study investigated the preva- lence of dengue virus (DENV) serotypes in the wild Aedes aegypti and their blood sources to determine human exposure risk in Dar es Salaam, Tanzania. A two-year longitudinal survey was conducted in the Ilala, Kinondoni, and Temeke districts of Dar es Salaam to sample Ae. aegypti mosquitoes using Biogents Sentinel trap (BGS), Prokopack aspira- tion, and Gravid Aedes trap (GAT). Collected mosquitoes were pooled in groups of 10 and tested for DENV1–4 serotypes using reverse transcription polymerase chain reaction (RT-qPCR). Blood meal sources were identified using an enzyme-linked immunosorbent assay (ELISA). Of 854 tested pools, only DENV-2 was detected and was found in all three districts: Temeke (3/371 pools), Ilala (1/206 pools), and Kinondoni (1/277 pools). Blood meal analysis showed a strong preference for humans (81%) as well as for mixed blood meals that contained human blood and other hosts (17%). Out of 354 collected hosts seeking Ae. aegypti, 78.5% were captured outdoors and 21.5% indoors. This study confirms the circulation of DENV-2 in Ae. aegypti populations, indicating a potential dengue outbreak risk in Tanzania. This study also demonstrates that xenomonitoring may be feasible in this setting. The mosquitoes’ strong preference for human hosts and predominance in outdoor settings pose challenges for dengue control effort
dc.identifier.urihttps://doi.org/10.3390/v17060818
dc.identifier.urihttps://dspace.nm-aist.ac.tz/handle/123456789/3289
dc.language.isoen
dc.publisherMDPI
dc.subjectDENV
dc.subjectDengue fever
dc.subjectTraps
dc.subjectBlood feeding
dc.subjectLongitudinal survey
dc.subjectSerotypes
dc.subjectXenomonitoring
dc.titleDENV-2 Circulation and Host Preference Among Highly Anthropophilic, Outdoor-Biting Aedes aegypti in Dar es Salaam, Tanzania
dc.typeArticle

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