Browsing by Author "Vanschoenwinkel, Bram"

Now showing 1 - 13 of 13

Culex quinquefasciatus mosquito avoids ovipositing in habitats with the annual fish (Nothobranchius neumanni) in Tanzania
(Springer Netherlands, 2024-05-03) Mataba, Gordian; Munishi, Linus; Brendonck, Luc; Vanschoenwinkel, Bram
Mosquitoes are known to choose oviposition habitats actively based on their suitability for survival of their offspring. They thus avoid laying eggs in dangerous habitats with aquatic predators through detection of their cues. Visual, tactile, and chemical cues emitted by certain predators have been shown to reduce oviposition in some mosquitoes, which in turn may affect their adult population sizes. However, those tests tend to be limited to species from temperate regions. Moreover, mosquito oviposition response to predator cues have been shown to be predator specific, but this has not been investigated for many potential predators worldwide. In the African subtropics, temporary ponds can be important mosquito breeding habitats and the same habitats are also used by a group of specialized killifish that can survive dry periods using drought resistant eggs. Although they are reported as mosquito predators, it is unknown whether mosquitoes actively avoid laying eggs in ponds with annual killifish. We investigated this using the mosquito Culex quinquefasciatus and the free and caged killifish Nothobranchius neumanni in the outdoor mesocosm experiments in Tanzania. We found that Cx. quinquefasciatus had 71% lower oviposition in mesocosms with free swimming killifish and 68% lower oviposition in mesocosms with caged killifish. We conclude that predator-released chemical cues alone are enough to explain the observed responses. If the cues can be isolated and chemically identified can be developed into a biological mosquito control strategy and used to safeguard public health in areas where mosquito borne diseases are endemic.
Environmental risks of a commonly used pyrethroid: Insights from temporary pond species of the Lake Manyara Basin, Tanzania
(Elsevier, 2023-01) Kafula, Yusuph; Thoré, Eli; Philippe, Charlotte; Munishi, Linus; Moyo, Francis; Vanschoenwinkel, Bram; Brendonck, Luc
Environmental risks posed by widespread pesticide application have attracted global attention. Currently, chemical risk assessments in aquatic environments rely on extrapolation of toxicity data from classic model species. However, similar assessments based on local species could be complementary, particularly for unusual living environments such as temporary ponds. Here, we carried out an environmental risk assessment (ERA) of a pyrethroid model compound, cypermethrin, based on local temporary pond species. First, we measured cypermethrin residue concentrations in rivers, irrigation canals and temporary ponds in the Lake Manyara Basin (LMB). Then, we estimated the environmental risks of cypermethrin by combining these data with acute toxicity data of three resident species across three trophic levels: primary producers (Arthrospira platensis), invertebrate grazers (Streptocephalus lamellifer) and fish (Nothobranchius neumanni). Furthermore, we compared the derived ERA to that obtained using toxicity data from literature of classic model species. Cypermethrin residue concentrations in contaminated systems of the LMB ranged from 0.01 to 57.9 ng/L. For temporary pond species, S. lamellifer was the most sensitive one with a 96 h-LC50 of 0.14 ng/L. Regardless of the assumed exposure concentration (0.01 and 57.9 ng/L), the estimated risks were low for primary producers and high for invertebrate grazers, both for local species as well as for classic model species. The highest detected cypermethrin concentration resulted in a moderate risk estimation for local fish species, while the estimated risk was high when considering classic fish models. Our results confirm that, at least for pyrethroids, ERAs with classic model species are useful to estimate chemical risks in temporary pond ecosystems, and suggest that complementary ERAs based on local species could help to fine-tune environmental regulations to specific local conditions and conservation targets.
Environmental risks of a commonly used pyrethroid: Insights from temporary pond species of the Lake Manyara Basin, Tanzania
(Elservier, 2023-04-10) Kafula, Yusuph; Thoré, Eli; Philippe, Charlotte; Munishi, Linus; Moyo, Francis; Vanschoenwinkel, Bram; Brendonck, Luc
Environmental risks posed by widespread pesticide application have attracted global attention. Currently, chemical risk assessments in aquatic environments rely on extrapolation of toxicity data from classic model species. However, similar assessments based on local species could be complementary, particularly for unusual living environments such as temporary ponds. Here, we carried out an environmental risk assessment (ERA) of a pyrethroid model compound, cypermethrin, based on local temporary pond species. First, we measured cypermethrin residue concentrations in rivers, irrigation canals and temporary ponds in the Lake Manyara Basin (LMB). Then, we estimated the environmental risks of cypermethrin by combining these data with acute toxicity data of three resident species across three trophic levels: primary producers (Arthrospira platensis), invertebrate grazers (Streptocephalus lamellifer) and fish (Nothobranchius neumanni). Furthermore, we compared the derived ERA to that obtained using toxicity data from literature of classic model species. Cypermethrin residue concentrations in contaminated systems of the LMB ranged from 0.01 to 57.9 ng/L. For temporary pond species, S. lamellifer was the most sensitive one with a 96 h-LC50 of 0.14 ng/L. Regardless of the assumed exposure concentration (0.01 and 57.9 ng/L), the estimated risks were low for primary producers and high for invertebrate grazers, both for local species as well as for classic model species. The highest detected cypermethrin concentration resulted in a moderate risk estimation for local fish species, while the estimated risk was high when considering classic fish models. Our results confirm that, at least for pyrethroids, ERAs with classic model species are useful to estimate chemical risks in temporary pond ecosystems, and suggest that complementary ERAs based on local species could help to fine-tune environmental regulations to specific local conditions and conservation targets.
Fish predation affects invertebrate community structure of tropical temporary ponds, with downstream effects on phytoplankton that are obscured by pesticide pollution
(Elsevier Ltd., 2024-02-14) Kafula, Yusuph; Mataba, Gordian; Mwaijengo, Grite; Moyo, Francis; Munishi, Linus; Vanschoenwinkel, Bram; Brendonck, Luc; Thoré, Eli
Aquatic biota of tropical temporary ponds typically experience a wide range of stressors that can drive the structure and dynamics of natural communities. Particularly in regions with intense agricultural activity, aquatic biota may not only experience predation pressure but also stress from pesticides that inadvertently enter the ponds. We increasingly understand how these different sources of stress affect classic model taxa under controlled laboratory conditions, but how predators and pesticides may jointly affect pond invertebrate communities is still unclear, particularly for tropical systems. Here, we conducted an outdoor mesocosm experiment to study how fish predation combined with exposure to an environmentally relevant concentration of the commonly used insecticide cypermethrin (0.8 ng/L) affects the structure of invertebrate communities, and its potential effects on leaf litter decomposition and invertebrate grazing efficiency as measures of ecosystem functioning. A total of seven invertebrate taxa were recorded in the mesocosm communities. Fish predation effectively lowered the number of invertebrate taxa, with fish mesocosms being dominated by high densities of rotifers, associated with lower phytoplankton levels, but only when communities were not simultaneously exposed to cypermethrin. In contrast, cypermethrin exposure did not affect invertebrate community structure, and neither fish predation nor cypermethrin exposure affected our measures of ecosystem functioning. These findings suggest that predation by killifish can strongly affect invertebrate community structure of tropical temporary ponds, and that downstream effects on phytoplankton biomass can be mediated by exposure to cypermethrin. More broadly, we contend that a deeper understanding of (tropical) temporary pond ecology is necessary to effectively manage these increasingly polluted systems.
Influence of land use on the abundance and spatial distribution of mosquito larvae of the Anopheles gambiae complex in a malaria expansion area in northern Tanzania
(Springer International Publishing, 2024-04) Mataba, Gordian; Munishi, Linus; Brendonck, Luc; Vanschoenwinkel, Bram
In many countries, successful control of larval mosquito populations for malaria control is still hindered by poor knowledge of preferred breeding habitats and natural factors limiting larval abundance. In Africa, preferred breeding habitats for malaria mosquitoes vary regionally and for many regions, they are still not well known. Moreover, variables that limit abundance of larvae of malaria mosquitoes are still ambiguous and impacts of surrounding land use practices are unclear. To study this, we sampled mosquito larvae from 164 small ground habitats (SGHs) in the lake Manyara basin (LMB) in northern Tanzania, a malaria expansion area with diverse land use types. We found that Anopheles gambiae s.l. was the only malaria mosquito breeding in SGHs in the region and its abundance was positively affected by turbidity and proximity to human dwellings. SGHs appear to be the major breeding sites for An. gambiae s.l. in the LMB despite the presence of larger temporary ponds which support negligible numbers of this species. In this region, we recommend that control of malaria via control of population of larval An. gambiae s.l. should prioritize SGHs to optimize use of limited resources and avoid damage to the environment by targeting unimportant habitats.
Interactive effects of dragonfly larvae andBacillusthuringiensisvar.israelensison mosquito ovipositionand survival
(John Wiley & Sons, Inc., 2023-09-05) Mataba, Gordian; Clark, Nicholas; Kweka, Eliningaya; Munishi, Linus; Brendonck, Luc; Vanschoenwinkel, Bram
The biological larvicide Bacillus thuringiensis var. israelensis (Bti) is used worldwide to control reproduction of mosquitoes in freshwater habitats. However, its impact on the ecosystem including nontarget species is often unclear. In addition, it is unknown how Bti larvicide may interact with local mosquito predators to shape oviposition site selection of mosquitoes. We used an outdoor mesocosm experiment to investigate the effects of realistic concentrations of the bio-larvicide Bti on Culex oviposition, larval density, survivorship, and on densities of nontarget species. We also manipulated the complexity of the community by manipulating the presence of dragonfly larvae as a predator. Culex oviposition was unaffected by Bti but the larvicide effectively reduced larval density and survivorship in all treatments. Bti did not affect nontarget insects but stimulated phytoplankton density at the expense of lower herbivore density. The presence of dragonfly larvae in mesocosms did not reduce Culex oviposition or larval sensitivity to Bti. We conclude that Bti may effectively reduce the density and survivorship of Culex quinquefasciatus mosquitoes in this part of East Africa, but possibly at the cost of higher phytoplankton densities. Bti-treated mesocosms were not more or less attractive for mosquitoes, suggesting that its application would not alter their oviposition behavior in the field.
Keep your natural enemies close – native predators can maintain low mosquito densities in temporary ponds in a malaria expansion area in Northern Tanzania
(Elsevier, 2021-11-10) Mataba, Gordian; Kafula, Yusuph; Mwaijengo, Grite; Snoeks, Joren; Munishi, Linus; Brendonck, Luc; Vanschoenwinkel, Bram
Mosquitoes and the diseases they transmit are a global public health threat that affects most human populations. Mosquito abundances are strongly linked to the number of suitable larval habitats available. However, it is still not well understood how different land uses impact larval habitat quality in different parts of the world. We investigated links between land use practices and abundance of mosquito larvae in temporary ponds located in different land uses in a malaria expansion zone in Tanzania. Temporary ponds are often cited as important sources of mosquitoes but are also of substantial conservation concern being home to a unique fauna and flora. Often, they also have high socio-economic importance. Overall, encountered mosquito larval abundances were very low, both for the collected Anopheles gambiae s.l. and Culex quinquefasciatus. Although temporary ponds are important mosquito larval habitats in other parts of Africa, currently they are unlikely to be an important factor affecting the prevalence of mosquito borne diseases in the Manyara study region. Larval abundances in temporary ponds were higher in agricultural areas where more eutrophic conditions were found and minimal in residential areas. Overall, even though temporary ponds are often modified as rice paddies in the rainy season, they were in a good ecological condition and hosted a diverse assemblage of aquatic predators that likely prevented larval mosquitoes from reaching high densities. Maintaining this good condition by preventing pesticide and nutrient input as much as possible, can be important to prevent pond degradation that is likely to make these habitats more suitable for mosquitoes in the future.
Large branchiopod occurrence and community structure in relation to land-use types in temporary ponds of northern Tanzania
(Springer Nature Switzerland AG., 2023-02-14) Kafula, Yusuph; Mataba, Gordian; Mwaijengo, Grite; Lemmens, Pieter; Munishi, Linus; Moyo, Francis; Dube, Trevor; Vanschoenwinkel, Bram; Brendonck, Luc
Large branchiopods are a key component of the fauna of temporary ponds and play an important role in the functioning of these vulnerable ecosystems. Owing to the establishment of new settlements and agricultural expansion, temporary ponds in Tanzania are disappearing at an alarming rate whilst little is known about their diversity and ecology. We contrasted temporary ponds from a protected area with those in communal lands to detect associations between land-use types and large branchiopod community structure. Six large branchiopod species were collected, five of which have been previously reported from Southern Africa, whilst one turned out to be new to science: Streptocephalus manyarensis n.sp. Kafula and Brendonck (2023). The clam shrimp Cyzicus sp., fairy shrimps Streptocephalus lamellifer Thiele (1900) and S. bourquinii Hamer and Appleton (1993) were the most abundant and widely occurring. Variation in large branchiopod community structure was explained by the presence of Nothobranchius killifish and orthophosphate concentration. The large branchiopod community structure was different in settlement and protected areas. Our study on the occurrence and structure of large branchiopod communities in relation to land-use types serves as a base for formulation of guidelines and management tools to regulate land-use practices adjacent to temporary pond ecosystems.
Pesticide sensitivity of Nothobranchius neumanni, a temporary pond predator with a non-generic life-history
(Elsevier, 2022-03) Kafula, Yusuph; Philippe, Charlotte; Pinceel, Tom; Munishi, Linus; Moyo, Francis; Vanschoenwinkel, Bram; Brendonck, Luc; Thoré, Eli
Pesticides are crucial to improve agricultural productivity, but often adversely affect surrounding aquatic systems and their fauna. To determine the environmental risk of pesticides, routine ecotoxicological tests are performed on several organisms, including standard fish models. However, these typically do not include fish species from variable habitats and with non-generic life-histories. In particular, inhabitants from temporary ponds such as annual killifish are conventionally understood to be resilient to natural stressors which could translate to higher pesticide resistance or, alternatively, trade-off with their resistance to pesticides and render them more sensitive than classic fish models. Using standard exposure tests, we assessed short-term toxicity effects of two commonly used pesticides, Roundup and cypermethrin, on the annual killifish Nothobranchius neumanni, and compared its sensitivity with that of classic fish models. For Roundup, we found a 72 h-LC50 of 1.79 ± 0.11 mg/L, which is lower than the values reported for zebrafish, medaka, fathead minnow and rainbow trout, suggesting that N. neumanni is more sensitive to the compound. The opposite was true for cypermethrin, with a 72 h-LC50 of 0.27 ± 0.03 mg/L. However, these LC50-values do not deviate strongly from those reported for other fish species, supporting earlier findings in the congeneric N. furzeri that the sensitivity of annual killifish to pollutants is similar to that of classic fish models despite their assumed robustness to environmental stress.
The role of anthropogenic container habitats as mosquito oviposition habitats in rural settlements in northern Tanzania
(BioOne Digital Library, 2022-04-05) Mataba, Gordian; Munishi, Linus; Brendonck, Luc; Vanschoenwinkel, Bram
Abstract In many areas, the main sources of mosquito vectors are not natural habitats but small artificial water bodies that are provided unintentionally by humans. Such container habitats have been linked to outbreaks of dengue fever and other arboviral diseases. However, in many parts of the world the possible risks associated with container habitats have not been assessed. Here, we focused on a human population expansion area in northern Tanzania with a high incidence of dengue and other cases of high fever. We explored the importance of anthropogenic container habitats for mosquito production in the Lake Manyara Basin. We also assessed how biotic and physicochemical habitat characteristics limit mosquito abundance in containers. Results showed that Aedes aegypti (Linnaeus), vector of dengue and other arboviruses, and Culex quinquefasciatus (Say), vector of filarial worms, were the dominant mosquitoes ovipositing in large numbers in different containers. Old tires were the dominant and most productive container habitat for mosquitoes in the region. However, there were strong differences among villages, illustrating that the mosquito burden associated with container habitats varies locally. We concluded that in this region, removal of artificial container habitats could be a simple strategy to reduce the mosquito-mediated disease burden within the local population.
Seasonal variation in benthic macroinvertebrate assemblages and water quality in an Afrotropical river catchment, northeastern Tanzania
(Elsevier, 2020-05) Mwaijengo, Grite Nelson; Vanschoenwinkel, Bram; Dube, Trevor; Njau, Karoli; Brendonck, Luc
Population growth and economic development have resulted in increased water demands, threatening freshwater resources. In riverine ecosystems, continuous monitoring of the river quality is needed to follow up on their ecological condition in the light of water pollution and habitat degradation. However, in many parts of the world, such monitoring is lacking, and ecological indicators have not been defined. In this study, we assessed seasonal variation in benthic macroinvertebrate assemblages in a tropical river catchment in northeastern Tanzania, which currently experiencing an increase in agricultural activities. We examined the potential of in-stream environmental variables and land-use patterns to predict the river macroinvertebrate assemblages, and also identified indicator taxa linked to specific water quality conditions. Macroinvertebrate abundance, taxon richness and TARISS (Tanzania River Scoring System) score were higher in the dry season most likely due to higher surface runoff from agricultural land and poorer water quality in the wet season. In the wet season macro invertebrates seem to be limited by chlorophyll-a, oxygen and phosphorous while in the dry season, when water flow is lower, nitrogen and turbidity become important. Substrate composition was important in both seasons. Given the fact that different selective filters limit macroinvertebrate assemblages in both seasons, a complete picture of water quality can only be established by monitoring in both seasons. Riparian buffer zones may help to alleviate some of the observed negative effects of agricultural activities on the river system in the wet season while limiting irrigation return flows may increase water quality in the dry season.
Where does land use matter most? Contrasting land use effects on river quality at different spatial scales
(Elsevier, 2020-05-01) Mwaijengo, Grite; Msigwa, Anna; Njau, Karoli; Brendonck, Luc; Vanschoenwinkel, Bram
Understanding the influence of land-use activities on river quality has been a key focus of river monitoring programs worldwide. However, defining which land-use spatial scale is relevant remains elusive. In this study, therefore, we contrasted the influence of land use on river quality using three types of land-use estimators, namely circular buffers around a monitoring site, circular buffers upstream of the monitoring site and the entire watershed area upstream of the monitoring site. The land-use percentage compositions within the Usa-Kikuletwa River catchment in northeastern Tanzania were quantified using Landsat-8 satellite images with a maximum mapping resolution of 30 m. Redundancy analysis models and generalized linear models were used to evaluate the influence of land use on macroinvertebrate assemblages and physico-chemical water quality at different spatial scales in the dry and wet seasons. Overall, a substantial fraction of variation in physico-chemical water quality, macroinvertebrate taxon richness, Chao-1 and TARISS (Tanzania River Scoring System) score could be explained by land use of the entire watershed area upstream of the monitoring site in the dry and wet seasons. However, macroinvertebrate abundances showed strong links with more local land-use patterns within 100 m and 2 km radii. Circular buffers upstream of monitoring sites were more informative for macroinvertebrate assemblages than circular buffers around the monitoring sites. However, the latter did correlate well with physico-chemical water quality variables. Land-use variables correlated across spatial scales (i.e., 100 m up to 2 km radii), but not with the land use in the entire watershed area above the monitoring site. Our results indicate that physico-chemical water quality variables and macroinvertebrates may respond differently to land-uses at different scales. More importantly, our results illustrate that the choice regarding spatial land-use metrics can bias conclusions of environmental impact studies in river systems.
Where does land use matter most? Contrasting land use effects on river quality at different spatial scales
(Elsevier B.V., 2020-05-01) Mwaijengo, Grite Nelson; Msigwa, Anna; Njau, Karoli; Brendonck, Luc; Vanschoenwinkel, Bram
Understanding the influence of land-use activities on river quality has been a key focus of river monitoring programs worldwide. However, defining which land-use spatial scale is relevant remains elusive. In this study, therefore, we contrasted the influence of land use on river quality using three types of land-use estimators, namely circular buffers around a monitoring site, circular buffers upstream of the monitoring site and the entire watershed area upstream of the monitoring site. The land-use percentage compositions within the Usa-Kikuletwa River catchment in northeastern Tanzania were quantified using Landsat-8 satellite images with a maximum mapping resolution of 30 m. Redundancy analysis models and generalized linear models were used to evaluate the influence of land use on macroinvertebrate assemblages and physico-chemical water quality at different spatial scales in the dry and wet seasons. Overall, a substantial fraction of variation in physico-chemical water quality, macroinvertebrate taxon richness, Chao-1 and TARISS (Tanzania River Scoring System) score could be explained by land use of the entire watershed area upstream of the monitoring site in the dry and wet seasons. However, macroinvertebrate abundances showed strong links with more local land-use patterns within 100 m and 2 km radii. Circular buffers upstream of monitoring sites were more informative for macroinvertebrate assemblages than circular buffers around the monitoring sites. However, the latter did correlate well with physico-chemical water quality variables. Land-use variables correlated across spatial scales (i.e., 100 m up to 2 km radii), but not with the land use in the entire watershed area above the monitoring site. Our results indicate that physico-chemical water quality variables and macroinvertebrates may respond differently to land-uses at different scales. More importantly, our results illustrate that the choice regarding spatial land-use metrics can bias conclusions of environmental impact studies in river systems.