Browsing by Author "Swennen, Rony"

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Better nitrogen fertilizer management improved Mchare banana productivity and profitability in northern highlands, Tanzania
(MDPI, 2023-05-20) Meya, Akida; Swennen, Rony; Ndakidemi, Patrick; Mtei, Kelvin; Merckx, Roel
Declining land productivity is a major problem constraining banana (Musa spp.) production in Tanzania. Banana fruit yield consequently reaches only 15% of the potential, primarily due to inadequate soil nutrient replenishment. Improving farmers’ soil nutrient replenishment strategy in banana home gardens, which relies on applications of cattle manure only, by mixing with inorganic fertilizer resources can increase land productivity and can improve the overall profitability of banana production in the country. Experiments were conducted at Tarakea, Lyamungo, and Tengeru to determine the effects of organic fertilizer resources (animal manure and crop residue) and their combination with inorganic fertilizer resources on the productivity and profitability of Mchare banana production. Banana fruit yield differed significantly among the experimental sites, with drier areas of Tengeru recording, on average, 19.6 t ha−1 year−1 , while the more humid areas of Lyamungo recorded, on average, 39.3 ha−1 year−1 . Mchare banana plants grown under sole inorganic fertilizer produced significantly low yields (33.0 t ha−1 year−1 ) compared with those fertilized with cattle manure only, which lifted the yields to 38.8 t ha−1 year−1 , but the latter required more labor input. Soil nitrogen (N) fertilization via cattle manure + mineral fertilizer gave the highest average banana fruit yield (43.0 t ha−1 year−1 ) across the sites, and reduced fertilization costs by 32%. Subsequently, this integrated fertilization technique generated the highest average net benefits in all sites and both cropping cycles. Thus, the findings of this study form a basis to improve land productivity and profitability in banana-based home gardens in the study area by directing more labor input to good soil N management
Datasets from harmonised metabolic phenotyping of root, tuber and banana crop
(Elsevier Inc., 2022-03-12) Drapal, Margit; Perez-Fons, Laura; Price, Elliott; Amah, Delphine; Bhattacharjee, Ranjana; Heider, Bettina; Rouard, Mathieu; Swennen, Rony; Lopez-Lavalle, Luis Augusto Becerra; Fraser, Paul
Biochemical characterisation of germplasm collections and crop wild relatives (CWRs) facilitates the assessment of biological potential and the selection of breeding lines for crop improvement. Data from the biochemical characterisation of staple root, tuber and banana (RTB) crops, i.e. banana (Musa spp.), cassava (Manihot esculenta), potato (Solanum tuberosum), sweet potato (Ipomoea batatas) and yam (Dioscorea spp.), using a metabolomics approach is presented. The data support the previously published research article “Metabolite database for root, tuber, and banana crops to facilitate modern breeding in understudied crops” (Price et al., 2020) [1]. Diversity panels for each crop, which included a variety of species, accessions, landraces and CWRs, were characterised. The biochemical profile for potato was based on five elite lines under abiotic stress. Metabolites were extracted from the tissue of foliage and storage organs (tuber, root and banana pulp) via solvent partition. Extracts were analysed via a combination of liquid chromatography – mass spectrometry (LC-MS), gas chromatography (GC)-MS, high pressure liquid chromatography with photodiode array detector (HPLC-PDA) and ultra performance liquid chromatography (UPLC)-PDA. Metabolites were identified by mass spectral matching to in-house libraries comprised from authentic standards and comparison to databases or previously published literature.
Effect of seasonal drought on the agronomic performance of four banana genotypes (Musa spp.) in the east african highlands
(MDPI, 2020-12-23) Uwimana, Brigitte; Zorrilla-Fontanesi, Yasmín; van Wesemael, Jelle; Mduma, Hassan; Brown, Allan; Carpentier, Sebastien; Swennen, Rony
Banana (Musa spp.), a perennial (sub-)tropical crop, suffers from seasonal droughts, which are typical of rain-fed agriculture. This study aimed at understanding the effect of seasonal drought on early growth, flowering and yield traits in bananas grown in the East African highlands. A field experiment was set up in North Tanzania using four genotypes from different geographical origins and two different ploidy levels. The treatments considered were exclusively rain-fed versus rain supplemented with irrigation. Growth in plant girth and leaf area were promising traits to detect the early effect of water deficit. Seasonal drought slowed down vegetative growth, thus significantly decreasing plant girth, plant height and the number of suckers produced when compared to irrigated plants. It also delayed flowering time and bunch maturity and had a negative effect on yield traits. However, the results depended on the genotype and crop cycle and their interaction with the treatments. “Nakitengwa”, an East African highland banana (EAHB; AAA genome group), which is adapted to the region, showed sensitivity to drought in terms of reduced bunch weight and expected yield, while “Cachaco” (ABB genome group) showed less sensitivity to drought but had a poorer yield than “Nakitengwa”. Our study confirms that seasonal drought has a negative impact on banana production in East Africa, where EAHBs are the most predominant type of bananas grown in the region. We also show that a drought-tolerant cultivar not adapted to the East African highlands had a low performance in terms of yield. We recommend a large-scale screening of diploid bananas to identify drought-tolerant genotypes to be used in the improvement of locally adapted and accepted varieties.
End-user preferences for plantain food products in Nigeria and implications for genetic improvement
(Oxford University Press, 2020-09-28) Amah, Delphine; Stuart, Esmé; Mignouna, Djana; Swennen, Rony; Teeken, Béla
Plantain is an important food security crop for farming households in Nigeria. This study investigates the relative importance of plantain food products and their quality descriptors from the perspective of farmers in Southern-Nigeria, to inform end-user oriented and socially inclusive breeding strategies that drive hybrid adoption. Surveys were conducted in twelve rural communities in three states in the plantain belt of Nigeria, consisting of key informant interviews, sex-disaggregated focused group discussions and individual interviews. Dodo (fried plantains), boli (roasted plantains), boiled plantain, plantain with beans and porridge were the most common food products identified in the study areas. Fruit size, pulp texture (firmness/softness), colour, maturity stage and taste were identified as the most critical characteristics, with impact on quality of processed food products. There were significant differences between states regarding the importance of plantain food products, but little interstate and gender differences for fresh fruit and food product quality characteristics.
Filling the gaps in gene banks: Collecting, characterizing,and phenotyping wild banana relatives of Papua NewGuinea
(John Wiley and Sons Inc, 2020-11-20) Eyland, David; Breton, Catherine; Sardos, Julie; Kallow, Simon; Panis, Bart; Swennen, Rony; Paofa, Janet; Tardieu, François; Welcker, Claude; Janssens, Steven
Since natural habitats are disappearing fast, there is an urgent need to collect, characterize, and phenotype banana (Musa spp.) crop wild relatives to identify unique genotypes with specific traits that fill the gaps in our gene banks. We report on a collection mission in Papua New Guinea carried out in 2019. Seed containing bunches were collected from Musa peekelii ssp. angustigemma (N.W.Simmonds) Argent (3), M. schizocarpa N. W. Simmonds (4), M. balbisiana Colla (3), M. acuminata ssp. banksii (F. Muell.) Simmonds (14), M. boman Argent (3), M. ingens Simmonds (2), M. maclayi ssp. maclayi F.Muell. ex Mikl.-Maclay (1), and M. lolodensis Cheesman (1). This material, together with the seeds collected during a previous mission in 2017, form the basis for the development of a wild banana seed bank. For characterization and phenotyping, we focused on the most ubiquitous indigenous species of Papua New Guinea: M. acuminata ssp. banksii, the ancestor of most edible bananas. We calculated that the median genomic dissimilarity of the M. acuminata ssp. banksii accessions was 4% and that they differed at least 5% from accessions present in the International Transit Centre, the world's largest banana gene bank. High-throughput phenotyping revealed drought avoidance strategies with significant differences in root/shoot ratio, soil water content sensitivity, and response towards vapor pressure deficit (VPD). We deliver a proof of principle that the wild diversity is not yet fully covered in the gene banks and that wild M. acuminata ssp. banksii populations contain individuals with unique traits, useful for drought tolerance breeding programs.
Genotype x environment response of ‘matooke’ hybrids (Naritas) to pseudocercospora fijiensis, the cause of black sigatoka in banana
(MDPI, 2021-06-03) Kimunye, Janet; Jomanga, Kennedy; Tazuba, Anthony; Were, Evans; Viljoen, Altus; Swennen, Rony; Mahuku, George
Growing bananas resistant to Pseudocercospora fijiensis, the cause of black Sigatoka, is the preferred disease control strategy for resource-poor farmers. Banana breeding programs in east Africa have developed 27 Matooke hybrids (commonly known as NARITAs) with higher yields than local landraces. To assess the response of NARITA hybrids to P. fijiensis, 22 hybrids were evaluated under natural field conditions in four locations—Kawanda and Mbarara in Uganda, and Maruku, and Mitarula in Tanzania—between 2016 and 2018 for three crop cycles. Black Sigatoka was visually assessed and the area under the disease progress curve calculated for each plant over time. Significant differences (p < 0.001) were observed between genotypes, environments, and their interaction. The highest contributor to black Sigatoka severity (39.1%) was the environment, followed by the genotype (37.5%) and the genotype Χ environment interaction (GEI) (23.4%). NARITA 2, 7, 14, 21 and 23 were resistant and the most stable hybrids across locations. If other attributes such as the yield and taste are acceptable to end-users, these hybrids can be released to farmers in the region to replace highly susceptible landraces. Mitarula was identified as an ideal site for evaluating banana against black Sigatoka and should be used as a representative location to minimize costs of disease evaluations.
Maize stover transfers from maize fields to banana-based agroforestry homegardens and the corresponding nutrient flows in central-northern Tanzania
(Frontiers in Forests and Global Change, 2023-07-13) Meya, Akida; Ndakidemi, Patrick; Mtei, Kelvin; Swennen, Rony; Merckx, Roel
Maize (Zea mays) stover produced in the drier lowland areas on the volcanic foot slopes of Mount Kilimanjaro serves as an important additional fodder for smallholder zero-grazed dairy cattle farming in the humid highland areas of the region. The obtained cattle manure is used to replenish nutrients and manage soil fertility in banana-based agroforestry homegardens of the highlands, which contributes significantly to sustaining crop production, thus food security and the livelihoods of the community. The study aims to provide a preliminary evaluation of the amounts of stover removed from smallholder maize-based fields in the lowlands and the subsequent nutrient flows to the banana-based agroforestry homegardens of the highlands. In 54 smallholder maize fields selected at random from 11 villages in the Hai and Siha districts of the Kilimanjaro region, we conducted an agronomic survey to record the types and amounts of fertilizer used, plant spacing at harvest, and maize stover biomass yield. Most of the surveyed maize-based fields received inadequate nutrient inputs, which were limited to mineral nitrogen alone if any. Of the surveyed fields, 16.7% received recommended amounts of 74 kg N ha–1 by the Ministry of Agriculture, 46.3% sub-optimal amounts and 37.0% did not receive fertilizer at all. Plant density was only 50% of the recommended population density. While this was only a preliminary study on nutrient flows between maize and banana-based agroecosystems, our findings demonstrated that potassium exports via harvested banana bunches exceeded the inputs in the homegardens. We conclude that (i) soil fertility management techniques, preferably integrated soil fertility management, suitable for small-scale maize production in the lowlands should be formulated while optimizing plant density to increase production, (ii) maize-based agroforestry farming system adapted to drier conditions to deal with windstorm and soil erosion problems in the lowlands is urgently needed, and (iii) wider and more detailed research on nutrient flows between maize and banana-based agroecosystems should be conducted to document their respective soil nutrient balances and the sustainability of such practice.
Optimizing Soil Fertility Management Strategies to Enhance Banana Production in Volcanic Soils of the Northern Highlands, Tanzania
(MDPI, 2020-02-18) Meya, Akida; Ndakidemi, Patrick; Mtei, Kelvin; Swennen, Rony; Merckx, Roel
Banana is an important crop in high altitude areas of Tanzania, grown widely both as a food staple and as the main source of income. However, its production is constrained by low soil fertility, a result of gradual nutrient mining by the crop. Currently, soil fertility management in banana-based farming systems in the country relies mainly on applications of animal manure. However, the amount of manure produced in most farms is not enough to replenish soil fertility due to the small number of animals kept by smallholder resource-poor farmers who are the major producers in the country. Field experiments were conducted at three sites with varying soil types and contrasting weather conditions along the altitudinal gradients on the slopes of the volcanic mountains of Kilimanjaro and Meru, northern Tanzania to (1) investigate the effect of mineral nitrogen (mineral N) fertilizer applications on the growth and yield of Mchare banana (Musa spp., AA, a traditional East African highland cooking banana sub-group), at the four levels of 0, 77, 153, and 230 kg N ha−1 year−1 as a starter strategy to improve the current soil fertility management strategies, and (2) evaluate the effect of the combined use of inorganic and organic N sources on growth and banana fruit production as an alternative strategy to manage soil fertility and minimize animal manure requirements. The treatment factors were trial sites (Tarakea, Lyamungo, and Tengeru) as the main factor and N fertilization strategies (as urea alone, sole cattle manure, and in combination with urea, sole common bean (Phaseolus vulgaris L.) haulms as well as in combination with urea) as a sub factor. Bean haulms and cattle manure were applied each year for two years. Fertilization at 153 kg N ha−1 year−1 derived solely from urea significantly (p < 0.001) resulted in high yield increment of up to 42% relative to the control. However, the increase was highest (52%) with the same N dose derived from cattle manure in combination with urea at 50% substitution. Sole bean haulms resulted in a smaller yield increment, the same as the lowest N dose from the sole urea fertilization treatment. The study concludes that soil fertility management in smallholder banana-based farming systems should not solely rely on animal manure and mineral fertilizers.
Pollen Amount and Viability in Mchare and Selected Wild (AA) Banana (Musa acuminata) Genotypes: Prospects for Breeding
(HortScience, 2024-04-10) Bayo, Stanley; Massawe, Veronica; Ndakidemi, Patrick; Venkataramana, Pavithravani; Mlaki, Anna; Mduma, Hassan; Jomanga, Kennedy; Swennen, Rony; Brown, Allan
East African diploid cooking bananas, commonly called Mchare, are a staple crop for millions of subsistence farmers in Tanzania, particularly in the Pangani region in northern Tanzania. Several pathogens constrain Mchare production significantly and threaten food security. Sources of resistance to these pathogens have been identified; however, partial male and female sterility impedes successful resistance introgression, complicating the breeding process. Mchare cultivars are also the only known surviving representatives of a diploid banana subgroup that contributed unreduced gametes to many of the most widely grown and successful triploid dessert bananas (‘Cavendish’, ‘Gros-Michel’, ‘Silk’, and ‘Prata’). As such, they represent an essential intermediate step in the conventional improvement of bananas worldwide. We assess the amount and viability of pollen among Mchare and wild genotypes to identify the most fertile Mchare cultivars that can be used in conventional banana improvement. Pollen was collected from 14 banana genotypes for quantification and viability testing over 7 months, and the optimal time for pollen collection was determined to be 0800 HR. Significant variation among banana genotypes in terms of both overall pollen production and percentage of pollen viability was observed. The wild-type bananas ‘Calcutta 4’ [International Musa Germplasm Transit Center (ITC) 0249] and ‘Borneo’ (ITC0253) had the greatest overall pollen production (> 31,000 pollen grains/anther) and viability (∼74%), whereas ‘Ijihu Inkundu’ (ITC1460; Mchare genotype) was the least productive (almost completely sterile), with an average pollen production of a few hundred grains per anther and a viability of 7%. There were significant differences among months in terms of pollen viability, with the greatest average viability observed in May, April, and February (> 51%), and the lowest average pollen viability in July (41%). Significant differences were observed among the Mchare genotypes, with ‘Huti-White’, ‘Huti green bell’ (ITC1559), and ‘Mchare Laini’ consistently producing more substantial amounts of total pollen and an overall more significant proportion of viable pollen. This information is vital to improve Mchare bananas and the global breeding of dessert bananas. The choice of Mchare banana used in improvement programs could affect fertility and the likelihood of breeding success.
Unravelling the complex story of intergenomic recombination in ABB allotriploid bananas
(Oxford University Press, 2021-01-01) Cenci, Alberto; Sardos, Julie; Hueber, Yann; Martin, Guillaume; Breton, Catherine; Roux, Nicolas; Swennen, Rony; Carpentier, Sebastien; Rouard, Mathieu
Bananas (Musa spp.) are a major staple food for hundreds of millions of people in developing countries. The cultivated varieties are seedless and parthenocarpic clones of which the ancestral origin remains to be clarified. The most important cultivars are triploids with an AAA, AAB or ABB genome constitution, with A and B genomes provided by M. acuminata and M. balbisiana, respectively. Previous studies suggested that inter-genome recombinations were relatively common in banana cultivars and that triploids were more likely to have passed through an intermediate hybrid. In this study, we investigated the chromosome structure within the ABB group, composed of starchy cooking bananas that play an important role in food security. Methods Using SNP markers called from RADSeq data, we studied the chromosome structure of 36 ABB genotypes spanning defined taxonomic subgroups. To complement our understanding, we searched for similar events within nine AB hybrid genotypes. Key Results Recurrent homologous exchanges (HEs), i.e. chromatin exchanges between A and B subgenomes, were unravelled with at least nine founding events (HE patterns) at the origin of ABB bananas prior to clonal diversification. Two independent founding events were found for Pisang Awak genotypes. Two HE patterns, corresponding to genotypes Pelipita and Klue Teparod, show an over-representation of B genome contribution. Three HE patterns mainly found in Indian accessions shared some recombined regions and two additional patterns did not correspond to any known subgroups. Conclusions The discovery of the nine founding events allowed an investigation of the possible routes that led to the creation of the different subgroups, which resulted in new hypotheses. Based on our observations, we suggest different routes that gave rise to the current diversity in the ABB cultivars, routes involving primary AB hybrids, routes leading to shared HEs and routes leading to a B excess ratio. Genetic fluxes took place between M. acuminata and M. balbisiana, particularly in India, where these unbalanced AB hybrids and ABB allotriploids originated, and where cultivated M. balbisiana are abundant. The result of this study clarifies the classification of ABB cultivars, possibly leading to the revision of the classification of this subgroup.
Using seminatural and simulated habitats for seed germination ecology of banana wild relatives
(John Wiley and Sons Ltd, 2021-10-10) Kallow, Simon; Quaghebeur, Katrijn; Panis, Bart; Janssens, Steven; Dickie, John; Gueco, Lavernee; Swennen, Rony; Vandelook, Filip
Ecologically meaningful seed germination experiments are constrained by access to seeds and relevant environments for testing at the same time. This is particularly the case when research is carried out far from the native area of the studied species. Here, we demonstrate an alternative—the use of glasshouses in botanic gardens as simulated-natural habitats to extend the ecological interpretation of germination studies. Our focal taxa were banana crop wild relatives (Musa acuminata subsp. burmannica, Musa acuminata subsp. siamea, and Musa balbisiana), native to tropical and subtropical South-East Asia. Tests were carried out in Belgium, where we performed germination tests in relation to foliage-shading/exposure to solar radiation and seed burial depth, as well as seed survival and dormancy release in the soil. We calibrated the interpretation of these studies by also conducting an experiment in a seminatural habitat in a species native range (M. balbisiana—Los Baños, the Philippines), where we tested germination responses to exposure to sun/shade. Using temperature data loggers, we determined temperature dynamics suitable for germination in both these settings. In these seminatural and simulated-natural habitats, seeds germinated in response to exposure to direct solar radiation. Seed burial depth had a significant but marginal effect by comparison, even when seeds were buried to 7 cm in the soil. Temperatures at sun-exposed compared with shaded environments differed by only a few degrees Celsius. Maximum temperature of the period prior to germination was the most significant contributor to germination responses and germination increased linearly above a threshold of 23℃ to the maximum temperature in the soil (in simulated-natural habitats) of 35℃. Glasshouses can provide useful environments to aid interpretation of seed germination responses to environmental niches.