Browsing by Author "Mwanyika, Gaspary"

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Bacterial contamination of pork carcasses from Arusha, Tanzania
(Global Journal of Advanced Research, 2016-09-30) Luanda, Catherine; Buza, Joram; Mwanyika, Gaspary; Lyimo, Beatus; Mrutu, Rehema
Consumer demand for pork in Tanzania is driving growth of an increasing number of poorly equipped formal and informal slaugther facilities. To assess the potential public health challenges from these developments, we assessed the magnitude of microbial contamination of pork carcases from slaughterhouses in urban Arusha. Carcasses surface swabs (n = 90) from three slaughterhouses (designated A, B and C with carcass turnover of 1-30, 30-50 and 50-100 per day, respectively) were sampled and E. coli and Salmonella isolates (n = 1,632 and n = 177, respectively) were tested against a panel of 11 antibiotics using breakpoint assays. On average, E. coli and Salmonella were recovered from 71.1% and 66.7% of carcasses, respectively. Prevalence was highest (E. coli, 90.0% and Salmonella, 90.0%) for carcasses from site B, a facility where all slaughter operations, including eviscerations, were completed on the floor. The lowest prevalence (E. coli, 43.3% and Salmonella, 36.7%) was recorded for carcasses from Site C, a facility where most operations were completed with the carcasses hanging from overhead rails. Resistance to at least one antibiotic was observed in 71.2% of the E. coli isolates, most commonly to ampicillin (53.2%) followed by amoxicillin (38.8%) and sulfamethoxazole (31.0%). Approximately 45.2% of Salmonella isolates were resistant to at least one antibiotic while 23.2% were resistant to two or more of the tested antibiotics. Resistance to streptomycin (19.8%), trimethoprim (18.6%), ampicillin (16.9%) and sulfamethoxazole (13.6%) were most common. All isolates were susceptible to cefotaxime, ceftazidime, and chloramphenicol. These findings associate poor slaughter practices with bacterial carcass contamination and provide evidence for potential to contract antimicrobial resistant E. coli and Salmonella.. General Terms: Microbial load, multidrug resist
Comparison of the prevalence of antibiotic-resistant Escherichia coli isolates from commercial-layer and free-range chickens in Arusha district, Tanzania
(African Journal of Microbiology Research, 2016-09-14) Rugumisa, Bernadether; Call, Douglas; Mwanyika, Gaspary; Subbiah, Murugan; Buza, Joram
The antibiotic susceptibility of fecal Escherichia coli isolates from commercial-layer and free-range chickens in Arusha district, Tanzania were compared. All the chickens were raised by individual households, but commercial-layer chickens were purchased from commercial vendors, whereas no systematic breeding system was used to produce free-range chickens. A total of 1,800 E. coli isolates (1,200 from commercial-layer chickens and 600 from free-range chickens) were tested for susceptibility to 11 antibiotics by breakpoint assays. All E. coli isolates were susceptible to gentamicin, ceftazidime and cefotaxime. Isolates from commercial-layer chickens had a high prevalence of resistance (32.4- 74.5%) for amoxicillin, ampicillin, ciprofloxacin, tetracycline, streptomycin, trimethoprim and sulfamethoxazole, while the prevalence of resistance to these antibiotics was lower (7-31.5%) for freerange chickens (P<0.05). Both groups had a similar prevalence of resistance to chloramphenicol (1.17- 1.5%; P>0.05). For antibiotic resistant strains, 64.1 and 91.5% of free-range and commercial-layer isolates, respectively, were resistant to ≥ 2 antibiotics. Commercial-layer chickens harbored significantly more resistant E. coli isolates (P<0.001) than free-range chickens, consistent with more exposure to antibiotics when compared with free-range chickens. Efforts should be directed towards motivating household owners to limit the use of antibiotics when they are investing in these breeds.
Load and Prevalence of Antimicrobial-Resistant Escherichia coli from Fresh Goat Meat in Arusha, Tanzania
(Journal of Food Protection, 2016) Mwanyika, Gaspary; Call, Douglas; Rugumisa, Bernadether; Luanda, Catherine; Mrutu, Rehema; Subbiah, Murugan; Buza, Joram
Given the potential public health risks associated with a burgeoning goat meat industry in Tanzania, we estimated the load of Escherichia coli and the prevalence of antibiotic-resistant strains for goat meat by using a cross-sectional study design (June to July 2015). Five large (n¼60 samples) and five small (n¼64 samples) slaughterhouses were sampled over a period of four to six visits each. Meat rinsate was prepared and plated onto MacConkey agar, and presumptive E. coli colonies were enumerated and reported as CFU per milliliter of rinsate. In total, 2,736 presumptive E. coli isolates were tested for antibiotic drug sensitivity by using breakpoint assays against 11 medically important antibiotics. E. coli was recovered from almost all the samples (96.8%), with counts ranging from 2 to 4 log CFU ml 1, and there was no significant difference (P¼0.43) in recovery according to facility size (average, 3.37 versus 3.13 log CFU ml 1, large and small, respectively). Samples from large facilities had relatively higher prevalence (P ¼ 0.026) of antibiotic-resistant E. coli compared with small facilities. This was mostly explained by more ampicillin (30.1 versus 12.8%) and amoxicillin (17.6 versus 4.5%) resistance for large versus small facilities, respectively, and more tetracycline resistance for small facilities (5.6 versus 10.6%, respectively). Large slaughter operations may serve as foci for dissemination of antibiotic-resistant bacteria via food products. More effective hygiene practices during slaughter and meat handling would limit the probability of transmitting antibiotic-resistant E. coli in goat meat.
Prevalence of Antibiotic-Resistant Fecal Escherichia coli Isolates from Penned Broiler and Scavenging Local Chickens in Arusha, Tanzania
(Journal of Food Protection, 2016) Rugumisa, Bernadether; Call, Douglas; Mwanyika, Gaspary; Mrutu, Rehema; Luanda, Catherine; Lyimo, Beatus; Subbiah, Murugan; Buza, Joram
We compared the prevalence of antibiotic-resistant Escherichia coli isolates from household-level producers of broiler (commercial source breeds) and local chickens in the Arusha District of Tanzania. Households were composed of a single dwelling or residence with independent, penned broiler flocks. Free-range, scavenging chickens were mixed breed and loosely associated with individual households. A total of 1,800 E. coli isolates (1,200 from broiler and 600 from scavenging local chickens) from 75 chickens were tested for their susceptibility against 11 antibiotics by using breakpoint assays. Isolates from broiler chickens harbored a higher prevalence of antibiotic-resistant E. coli relative to scavenging local chickens, including sulfamethoxazole (80.3 versus 34%), followed by trimethoprim (69.3 versus 27.7%), tetracycline (56.8 versus 20%), streptomycin (52.7 versus 24.7%), amoxicillin (49.6 versus 17%), ampicillin (49.1 versus 16.8%), ciprofloxacin (21.9 versus 1.7%), and chloramphenicol (1.5 versus 1.2%). Except for resistance to chloramphenicol, scavenging local chickens harbored fewer resistant E. coli isolates (P , 0.05). Broiler chickens harbored more isolates that were resistant to 7 antibiotics (P , 0.05). The higher prevalence of antibiotic-resistant E. coli from broiler chickens correlated with the reported therapeutic and prophylactic use of antibiotics in this poultry population. We suggest that improved biosecurity measures and increased vaccination efforts would reduce reliance on antibiotics by these households.
Recovery and prevalence of antibiotic-resistant Salmonella from fresh goat meat in Arusha, Tanzania
(African Journal of Microbiology Research, 2016-08-28) Mwanyika, Gaspary; Buza, Joram; Rugumisa, Benardether; Luanda, Catherine; Murutu, Rehema; Lyimo, Beatus; Subbiah, Murugan; Call, Douglas
Meat products are clearly associated with foodborne pathogens including, antibiotic-resistant strains. Population growth and growing consumer demand facilitate the transmission of foodborne pathogens, particularly in developing countries. To determine the prevalence of antibiotic-resistant Salmonella in goat meat, a study was done in Tanzania (June to July, 2015). Overall 120 goat meat samples were collected from five large and five small slaughter facilities (n = 60, respectively). Pre-enrichment for Salmonella isolation was done in Tryptic Soy Broth followed by selective enrichment in Modified Semisolid Rappaport-Vassiliadis agar. Isolation of Salmonella was done in xylose-Lysine-Deoxycholate agar followed by biochemical confirmation in triple sugar iron agar. The average prevalence of Salmonella was 60 and 63% in large and small facilities, respectively. Breakpoint assays indicated an overall low prevalence of resistance (2 to 4%; n = 219 isolates) to ampicillin, amoxicillin, streptomycin, sulphamethoxazole and trimethoprim with complete susceptibility to ciprofloxacin, ceftazidime and cefotaxime. No significant difference (p > 0.05) in the prevalence of resistance between large and small facilities was observed. High probability of Salmonella contamination of goat meat from Arusha area of Tanzania can pose risks to consumers. Antibiotic resistance appears minimal in this population. Improved hygienic slaughter and meat-handling practices are encouraged to reduce the burden of Salmonella-positive meat products.