Browsing by Author "McGiven, John"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Performance characteristics and costs of serological tests for brucellosis in a pastoralist community of northern Tanzania
    (Springer Nature Limited, 2021-03-09) Lukambagire, AbdulHamid; Mendes, Ângelo; Bodenham, Rebecca; McGiven, John; Mkenda, Nestory; Mathew, Coletha; Rubach, Matthew; Sakasaka, Philoteus; Shayo, Davis; Maro, Venance; Shirima, Gabriel; Kasanga, Christopher; Kazwala, Rudovick; Halliday, Jo; Mmbaga, Blandina
    The control of brucellosis across sub-Saharan Africa is hampered by the lack of standardized testing and the use of tests with poor performance. This study evaluated the performance and costs of serological assays for human brucellosis in a pastoralist community in northern Tanzania. Serum collected from 218 febrile hospital patients was used to evaluate the performance of seven index tests, selected based on international recommendation or current use. We evaluated the Rose Bengal test (RBT) using two protocols, four commercial agglutination tests and a competitive enzyme-linked immunosorbent assay (cELISA). The sensitivity, specificity, positive predictive value, negative predictive value, Youden’s index, diagnostic accuracy, and per-sample cost of each index test were estimated. The diagnostic accuracy estimates ranged from 95.9 to 97.7% for the RBT, 55.0 to 72.0% for the commercial plate tests, and 89.4% for the cELISA. The per-sample cost range was $0.69–$0.79 for the RBT, $1.03–$1.14 for the commercial plate tests, and $2.51 for the cELISA. The widely used commercial plate tests performed poorly and cost more than the RBT. These findings provide evidence for the public health value of discontinuing the use of commercial agglutination tests for human brucellosis in Tanzania.
  • Thumbnail Image
    Item
    Prevalence and speciation of brucellosis in febrile patients from a pastoralist community of Tanzania.
    (Springer Nature Limited, 2020-04-27) Bodenham, Rebecca; Lukambagire, Abdul; Ashford, Roland; Buza, Joram; Cash-Goldwasser, Shama; Crump, John; Kazwala, Rudovick; Maro, Venance; McGiven, John; Mkenda, Nestory; Mmbaga, Blandina; Rubach, Matthew; Sakasaka, Philoteus; Shirima, Gabriel; Swai, Emanuel; Thomas, Kate; Whatmore, Adrian; Haydon, Daniel; Halliday, Jo
    Brucellosis is an endemic zoonosis in sub-Saharan Africa. Pastoralists are at high risk of infection but data on brucellosis from these communities are scarce. The study objectives were to: estimate the prevalence of human brucellosis, identify the Brucella spp. causing illness, describe non-Brucella bloodstream infections, and identify risk factors for brucellosis in febrile patients from a pastoralist community of Tanzania. Fourteen (6.1%) of 230 participants enrolled between August 2016 and October 2017 met study criteria for confirmed (febrile illness and culture positivity or ≥four-fold rise in SAT titre) or probable (febrile illness and single SAT titre ≥160) brucellosis. Brucella spp. was the most common bloodstream infection, with B. melitensis isolated from seven participants and B. abortus from one. Enterococcus spp., Escherichia coli, Salmonella enterica, Staphylococcus aureus and Streptococcus pneumoniae were also isolated. Risk factors identified for brucellosis included age and herding, with a greater probability of brucellosis in individuals with lower age and who herded cattle, sheep or goats in the previous 12 months. Disease prevention activities targeting young herders have potential to reduce the impacts of human brucellosis in Tanzania. Livestock vaccination strategies for the region should include both B. melitensis and B. abortus.