Browsing by Author "Makumba, Billy"

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Advancement and practical applications of rhizobacterial biofertilizers for sustainable crop production in sub-Saharan Africa
(BioMed Central, 2021-12-13) Aloo, Becky; Mbega, Ernest; Tumuhairwe, John; Makumba, Billy
Agricultural intensification continues in Africa in attempts to meet the rising food demands of the equally rising population. However, most arable lands in the region are characterized by nutrient deficiency and over-reliance on synthetic fertilizers which consequently contributes to increased production costs, environmental pollution, and global warming. Decades of research on plant–rhizobacterial interactions have led to the formulation and commer- cialization of rhizobacterial biofertilizers globally for sustainable soil and crop health. Nevertheless, this promising technology has not received much attention in Africa and remains largely unexplored due to several constraints. This article discusses the practical applications of rhizobacterial biofertilizers for sustainable crop production in sub-Saha- ran Africa. The challenges of soil infertility and the use of conventional synthetic fertilizers in crop production in Africa are critically evaluated. An overview of the potential of rhizobacteria as biofertilizers and alternatives to synthetic fertilizers for soil fertility and crop productivity in the continent is also provided. The advantages that these biofertiliz- ers present over their synthetic counterparts and the status of their commercialization in the African region are also assessed. Finally, the constraints facing their formulation, commercialization, and utilization and the prospects of this promising technology in the region are deliberated upon. Such knowledge is valuable towards the full exploitation and adoption of this technology for sustainable agriculture for Africa’s food security.
Effects of agrochemicals on the beneficial plant rhizobacteria in agricultural systems
(Springer Nature, 2021-09-18) Aloo, Becky; Mbega, Ernest; Makumba, Billy; Tumuhairwe, John
Conventional agriculture relies heavily on chemical pesticides and fertilizers to control plant pests and diseases and improve production. Nevertheless, the intensive and prolonged use of agrochemicals may have undesirable consequences on the structure, diversity, and activities of soil microbiomes, including the beneficial plant rhizobacteria in agricultural systems. Although literature continues to mount regarding the effects of these chemicals on the beneficial plant rhizobacteria in agricultural systems, our understanding of them is still limited, and a proper account is required. With the renewed efforts and focus on agricultural and environmental sustainability, understanding the effects of different agrochemicals on the beneficial plant rhizobacteria in agricultural systems is both urgent and important to deduce practical solutions towards agricultural sustainability. This review critically evaluates the effects of various agrochemicals on the structure, diversity, and functions of the beneficial plant rhizobacteria in agricultural systems and propounds on the prospects and general solutions that can be considered to realize sustainable agricultural systems. This can be useful in understanding the anthropogenic effects of common and constantly applied agrochemicals on symbiotic systems in agricultural soils and shed light on the need for more environmentally friendly and sustainable agricultural practices.
Effects of Carrier Materials and Storage Temperatures on the Viability and Stability of Three Biofertilizer Inoculants Obtained from Potato (Solanum tuberosum L.) Rhizosphere
(MDPI, 2022-01-20) Aloo, Becky; Mbega, Ernest; Makumba, Billy; Tumuhairwe, John
Biofertilizer technology continues to be derailed by the short shelf life of inoculants. The present study investigated the suitability of wheat-bran (WB), rice-husks (RH), farmyard-manure (FYM), bagasse (BG), and sawdust (SD) in the formulation of potato-derived Klebsiella grimontii (MPUS7), Serratia marcescens (NGAS9), and Citrobacter freundii (LUTT5) under refrigerated (8 °C) and room (25 ± 2 °C) storage. The physicochemical properties of the materials were assessed before sterilization and introduction of the inoculants and assessment of their viability for 8 months. Most of the physicochemical properties of the materials varied significantly (p < 0.05). Bagasse supported the maximum growth of MPUS7 (5.331 log CFU g−1) under refrigeration and LUTT5 (4.094 log CFU g−1) under both conditions. Under room storage, the maximum growth of MPUS7 (3.721 log CFU g−1) occurred in WB. Formulations that remained viable under room storage can easily be integrated into existing agricultural distribution systems that lack refrigeration.
Molecular Identification and In Vitro Plant Growth-Promoting Activities of Culturable Potato (Solanum tuberosum L.) Rhizobacteria in Tanzania
(Springer Nature Switzerland AG., 2020-08-21) Aloo, Becky; Mbega, Ernest; Makumba, Billy; Hertel, Robert; Daniel, Rolf
The present study investigated the diversity of culturable rhizobacteria associated with potato (S. tuberosum L.) in Tanzania and assessed their in vitro plant growth-promoting (PGP) activities to deduce their potential as biofertilizers. Potato rhizosphere soil and tuber samples (54 samples in total) were collected from 9 villages in three different agro-ecological regions in Tanzania. A total of 145 rhizobacterial isolates were obtained, 52 of which were selected and identified by partial 16S rRNA gene sequences and screened for various PGP traits in vitro including qualitative and quantitative solubilization of phosphorus (P), zinc (Zn) and potassium (K), nitrogen (N2) fixation and production of ammonia (NH3) in nitrogen-free medium, and indole-3-acetic acid (IAA), gibberellic acids (GA) and siderophores production. The results showed that the isolates were all Gammaproteobacteria, belonging to 4 families (Enterobacteriaceae, Yersiniaceae, Pseudomonadaceae and Morganellaceae) and 9 genera (Enterobacter, Klebsiella, Serratia, Pseudomonas, Morganella, Buttiauxella, Pantoea and Cedecea). Significant differences (P < 0.05) were observed for all assessed PGP abilities of the external and endophytic rhizobacterial isolates except for quantitative siderophore production and qualitative P and K solubilization for the external rhizobacteria and production of IAA and GA for the endophytic rhizobacteria. Among the best PGP isolates which can be exploited for biofertilization of the potato were Klebsiella pneumoniae KIBS1, K. grimontii LUTS10, Serratia liquefaciens KIBT1, Enterobacter ludwigii KIBS10 and Citrobacter freundii MWALS6. Comparative evaluation of PGP abilities of these two groups of isolates revealed significant differences (P < 0.05) only for NH3 and IAA production and qualitative K solubilization.
Nutrient Budgets for Sustained Crop Production in African Soils: Evidence from Potato-Grown Soils in Tanzania
(Springer Nature Singapore, 2024-11-12) Aloo, Becky; Tripathi, Vishal; Makumba, Billy; Mbega, Ernest
Soil fertility is under pressure worldwide due to agricultural intensification to match food demand. In sub-Saharan Africa (SSA), the depletion of soil nutrients is the root cause of the widespread food insecurity. Therefore, there is a need to continually monitor soil nutrient levels to drive policies and management options for sustained food production. Compared to other crops, potato (Solanum tuberosum L.) is increasingly becoming significant for food and economic security in SSA. The crop is, however, a heavy nutrient feeder and withdraws hefty amounts of nutrients from the soil which can interfere with its sustained production. However, very few studies have assessed the soil fertility levels of potato-grown soils in different parts of SSA. This chapter includes a case study report from the investigation of the fertility status and associated physicochemical properties of potato-grown soils in various agro-ecological areas in Tanzania. Soil samples collected from 27 potato farmlands were sampled in nine districts and analyzed for pH, electrical conductivity at 25 °C (EC25), soluble salts (SS), particle size distribution, potassium, phosphorus, iron, nitrogen, zinc, organic matter (OM), and organic carbon (OC) using standard methods. The results revealed significant differences for fertility-related physicochemical properties like EC25 (P = 0.004), (%) SS (P = 0.004), (%) OC (P = 0.018), and (%) OM (P = 0.019) in the soils but there were no significant differences for the fertility status of soils across the different study areas. Significant (P < 0.05) correlations were also observed for different parameters. The study concluded that specific soil characteristics differed significantly while fertility was relatively consistent. These findings serve as a basis for comprehending the existing soil conditions and can inform future strategies for sustainable soil management to ensure optimal nutrient levels to support the continued productivity of potatoes in the region.
Plant growth-promoting rhizobacterial biofertilizers for crop production: The past, present, and future
(Frontiers, 2022-09-16) Aloo, Becky; Tripathi, Vishal; Makumba, Billy; Mbega, Ernest
Recent decades have witnessed increased agricultural production to match the global demand for food fueled by population increase. Conventional agricultural practices are heavily reliant on artificial fertilizers that have numerous human and environmental health effects. Cognizant of this, sustainability researchers and environmentalists have increased their focus on other crop fertilization mechanisms. Biofertilizers are microbial formulations constituted of indigenous plant growth-promoting rhizobacteria (PGPR) that directly or indirectly promote plant growth through the solubilization of soil nutrients, and the production of plant growth-stimulating hormones and iron- sequestering metabolites called siderophores. Biofertilizers have continually been studied, recommended, and even successfully adopted for the production of many crops in the world. These microbial products hold massive potential as sustainable crop production tools, especially in the wake of climate change that is partly fueled by artificial fertilizers. Despite the growing interest in the technology, its full potential has not yet been achieved and utilization still seems to be in infancy. There is a need to shed light on the past, current, and future prospects of biofertilizers to increase their understanding and utility. This review evaluates the history of PGPR biofertilizers, assesses their present utilization, and critically advocates their future in sustainable crop production. It, therefore, updates our understanding of the evolution of PGPR biofertilizers in crop production. Such information can facilitate the evaluation of their potential and ultimately pave the way for increased exploitation.
The potential of Bacilli rhizobacteria for sustainable crop production and environmental sustainability.
(Elsevier, 2019-02-01) Aloo, Becky; Makumba, Billy; Mbega, Ernest
Conventional agricultural practices often rely on synthetic fertilizers and pesticides which have immense and adverse effects on humans, animals and environments. To minimize these effects, scientists world over are now deeply engaged in finding alternative approached for crop production which are less dependent on chemical inputs. One such approach is the use of rhizospheric bacteria as vital components of soil fertility and plant growth promotion (PGP) through their direct and indirect processes in plant rhizospheres. Among the most studied rhizobacteria are the Bacilli, particularly for production of antibiotics, enzymes and siderophores all of which are important aspects of PGP. Despite this, little information is available especially on their potentiality in crop production and their direct application only involves a few species, leaving a majority of these important rhizobacteria unexploited. This paper gives an overview of the unique properties of Bacilli rhizobacteria as well as their different PGP mechanisms that if mined can lead to their successful application and agricultural sustainability. It further points out the missing aspects with regards to these important rhizobacteria that should be considered for future research. This information will be useful in analyzing the PGP abilities of Bacilli rhizobacteria with an aim of fully mining their potential for crop production and environmental sustainability.
Status of biofertilizer research, commercialization, and practical applications: A global perspective
(Elsevier Inc., 2021) Aloo, Becky; Makumba, Billy; Mbega, Ernest
Most contemporary agricultural practices involve the use of synthetic fertilizers which have been linked to numerous deleterious consequences such as eutrophication of water bodies and emission of greenhouse gases. Biofertilizers offer viable and environmentally friendly alternatives. The positive effects of plant growth-promoting rhizobacteria have extensively been demonstrated several agronomically important crops under both controlled and field conditions. Despite the large volume of literature documenting the potential of these microbial inoculants as biofertilizers, their practical application has largely been hampered by several factors. This chapter presents the current knowledge of biofertilizer research, commercialization, and practical applications from the global perspective. The constraints facing their research and global application are also articulated. Finally, some prospects regarding their future research, commercialization and practical application for sustainable cropping systems are critically elucidated. It is anticipated that this will enable the full evaluation of the potential prospects of biofertilizers for sustainable agriculture and ecosystems globally.
Whole-Genome Sequences of Three Plant Growth-Promoting Rhizobacteria Isolated from Solanum tuberosum L. Rhizosphere in Tanzania.
(American Society for Microbiology, 2020-05-14) Aloo, Becky; Mbega, Ernest; Makumba, Billy; Friedrich, Ines; Hertel, Robert; Daniel, Rolf
We present here the complete genome sequences of plant growth-promoting sp. strain MPUS7, sp. strain NGAS9, and sp. strain LUTT5, isolated from rhizosphere soils and tubers of potato ( L.) plants growing in the northern and southern highlands of Tanzania.