Browsing by Author "Carvalho, Fernando"

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    Bacterial Diversity Dynamics in Sandy Loam Soils in Tanzania Under Varying Fertilizer-Derived Uranium Concentrations
    (Microorganisms, 2025-08-13) Mwalongo, Dennis; Lisuma, Jacob; Haneklaus, Nils; Maged, Ali; Brink, Hendrik; Carvalho, Fernando; Wacławek, Stanisław; Mpumi, Nelson; Amasi, Aloyce; Mwimanzi, Jerome; Chuma, Furaha; Kivevele, Thomas; Mtei, Kelvin
    The presence of radiotoxic uranium (U) in mineral fertilizers is of global concern. A pilot study was conducted in Tabora (Tanzania) to determine the release of U from three brands of phosphate fertilizers and its impact on soil bacteria. The experiment used three types of fertilizer: Minjingu Powder (MP), Nafaka Plus (NP), a mixed and granulated fertilizer made from Minjingu Phosphate Rock (MPR), and YaraMila Cereal (YC) fertilizer. There was also a control treatment that was not fertilized (NF). Alpha diversity and the R tool were used to analyze bacterial diversity in four samples within an average sequencing depth of 74,466 reads, using metrics like ASVs, Shannon index, and Chao1. The results showed that the number of amplicon sequence variants (ASVs) in the DNA from soil bacteria decreased, specifically to 400 ASVs, in the NP treatment, which was in line with the higher U concentration (3.93 mg kg−1) in the soils. In contrast, the MP fertilizer treatment, associated with a lower U concentration (3.06 mg kg−1) in soils, exhibited an increase in ASVs within the DNA of soil bacteria, reaching 795; the highest ASV value (822) was observed in the NF treatment. Higher amounts of U in the soil plots seemed to have resulted in more types of bacteria, with the Actinobacteriota phylum being the most common in all of the treatments. The NP (3.93 mg kg−3 U concentration) and MP (3.06 mg kg−3 U concentration) treatments were the only ones that showed Halobacteriota and Crenarchaeota phyla. Nonetheless, bacterial diversity may also account for the alterations in soil phosphorus and nitrogen following fertilizer application. The YaraMila Cereal treatment did not seem to be linked to any particular bacterial phylum. This means that in this study it did not have any measurable effect on the soil bacteria species compared to the MP and NP treatments
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    Influence of phosphate fertilizers on the radioactivity of agricultural soils and tobacco plants in Kenya, Tanzania, and Uganda
    (Springer Berlin Heidelberg, 2023-06-20) Mwalongo, Dennis; Haneklaus, Nils; Carvalho, Fernando; Lisuma, Jacob; Kivevele, Thomas; Mtei, Kelvin
    Three brands of NPK fertilizers that contain variable concentrations of natural radioactivity are commonly used in tobacco plantations in Kenya, Tanzania, and Uganda. Tobacco plants are known for hyper-accumulation of natural radionuclides, particularly 238U. This study investigated if the elevated radioactivity in phosphate fertilizers could enhance radioactivity in soils and tobacco plant leaves. The 232Th, 238U, and 40K radionuclide levels in NPK-fertilized soils and tobacco leaves were measured using gamma-ray spectroscopy. The research included a one-year reference experiment with tobacco growing in plots, a ten-year semi-controlled experiment in well-managed tobacco farms, and a field survey of radioactivity in soils and tobacco leaves at three traditional tobacco fields in Migori (Kenya), Urambo (Tanzania), and Kanungu (Uganda). The findings demonstrated that soils and tobacco leaves exposed to NPK fertilizers with increased radioactivity had activity concentrations of 232 Th, 238 U, and 40 K that were considerably higher (at all sites) than in the control samples (with no use of NPK fertilizers). As the continued application of NPK fertilizers raises concentrations of 232Th, 238U, and 40K in agricultural soils, the study assessed radiological risks for humans from exposure to agricultural soils enriched with phosphate fertilizers, and it was found to be below the exposure limit of 1 mSvy-1 suggested by the International Commission on Radiological Protection (ICRP). However, tobacco consumers, both by snuffing and smoking, may face significant radiological risks, as the snuffing and smoking resulted in effective doses that were 2.41 to 6.53 and 1.14 to 2.45 times greater than the average yearly dose that the general public receives from inhalation of natural radionuclides (United Nations Scientific Committee on Atomic Radiations estimates). Furthermore, the results indicate that the lifetime excess cancer risk for tobacco snuffers and smokers ranged from 5 × 10 -5 to 24.48 × 10 -3 and 2.0 × 10 -5 to 9.18 × 10 -3 , respectively. The influence of phosphorus-derived fertilizer containing relatively high natural radioactivity, potential human radiation exposure, and radiological risk due to gamma radionuclides is estimated and discussed. The results reveal that applying phosphate fertilizers enhances natural radioactivity in soil and is subsequently influenced by soil to tobacco plant uptake. Therefore, the study recommends that countries use fertilizers with lower radionuclide content to conserve soil quality and reduce gamma-emitting radionuclides in tobacco plants.