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Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity

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dc.contributor.author Nkabinde, L. A.
dc.contributor.author Shoba-Zikhali, L. N. N.
dc.contributor.author Semete-Makokotlela, B.
dc.contributor.author Kalombo, L.
dc.contributor.author Swai, Hulda
dc.contributor.author Grobler, A.
dc.contributor.author Hamman, J. H.
dc.date.accessioned 2020-08-31T07:29:39Z
dc.date.available 2020-08-31T07:29:39Z
dc.date.issued 2014
dc.identifier.uri https://doi.org/10.3144/expresspolymlett.2014.23
dc.identifier.uri https://dspace.nm-aist.ac.tz/handle/123456789/890
dc.description This research article published by eXPRESS Polymer Letters Vol.8, No.3 (2014) en_US
dc.description.abstract Nanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within the lysosomal compartment and their cytotoxicity in different cell lines. Rhodamine-6G labelled poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation freeze-drying method. Uptake and co-localisation of PLGA nanoparticles in lysosomes were visualized by confocal laser scanning microscopy. The cytotoxicity of the nanoparticles was evaluated on different mammalian cells lines by means of Trypan blue exclusion and the MTS assay. The PLGA nanoparticles accumulated in the intercellular spaces of Caco-2 cell monolayers, but were also taken up transcellularly into the Caco-2 cells and partially co-localized within the lysosomal compartment indicating involvement of endocytosis during uptake. PLGA nanoparticles did not show cytotoxic effects in all three cell lines. Intact PLGA nanoparticles are therefore capable of moving across epithelial cell membranes partly by means of endocytosis without causing cytotoxic effects. en_US
dc.language.iso en en_US
dc.publisher eXPRESS Polymer Letters en_US
dc.subject Biocompatible polymers en_US
dc.subject Caco-2 cells en_US
dc.subject Cellular uptake en_US
dc.subject PLGA nanoparticles en_US
dc.title Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity en_US
dc.type Article en_US


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