Diversity and distribution of Maize-associated totivirus strains from Tanzania.

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dc.contributor.author Read, David Alan
dc.contributor.author Featherston, Jonathan
dc.contributor.author Rees, David Jasper Gilbert
dc.contributor.author Thompson, Genevieve Dawn
dc.contributor.author Roberts, Ronel
dc.contributor.author Flett, Bradley Charles
dc.contributor.author Mashingaidze, Kingstone
dc.contributor.author Pietersen, Gerhard
dc.contributor.author Kiula, Barnabas
dc.contributor.author Kullaya, Alois
dc.contributor.author Mbega, Ernest R
dc.date.accessioned 2019-08-06T07:07:54Z
dc.date.available 2019-08-06T07:07:54Z
dc.date.issued 2019-06-01
dc.identifier.issn 1572-994X
dc.identifier.other 30790190
dc.identifier.uri https://doi.org/10.1007/s11262-019-01650-6
dc.identifier.uri http://dspace.nm-aist.ac.tz/handle/123456789/396
dc.description Research Article published by Springer en_US
dc.description.abstract Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, where it has been found to co-occur with other viruses known to elicit maize lethal necrosis disease (MLND). In a survey for maize-associated viruses, 35 samples were selected for Illumina HiSeq sequencing, from the Tanzanian maize producing regions of Mara, Arusha, Manyara, Kilimanjaro, Morogoro and Pwani. Libraries were prepared using an RNA-tag-seq methodology. Taxonomic classification of the resulting datasets showed that 6 of the 35 samples from the regions of Arusha, Kilimanjaro, Morogoro and Mara, contained reads that were assigned to MATV reference sequences. This was confirmed with PCR and Sanger sequencing. Read assembly of the six MATV-associated datasets yielded partial MATV genomes, two of which were selected for further characterization, using RACE. This yielded two full-length MATV genomes, one of which is divergent from other available MATV genomes. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.subject Illumina sequencing en_US
dc.subject Maize en_US
dc.subject Maize lethal necrosis disease en_US
dc.subject Tanzania en_US
dc.subject Totivirus en_US
dc.title Diversity and distribution of Maize-associated totivirus strains from Tanzania. en_US
dc.type Article en_US

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