Browsing Life sciences and Bio-engineering by Author "Gwakisa, Paul"
Now showing items 1-11 of 11
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16S rRNA Amplicons Survey Revealed Unprecedented Bacterial Community in Solid Biomedical Wastes
Mwaikono, Kilaza Samson; Maina, Solomon; Sebastian, Aswathy; Kapur, Vivek; Gwakisa, Paul (Science and Education Publishing, 2015)Despite known risks of inappropriate disposal of biomedical solid waste; most cities in developing countries are still disposing unsorted and untreated solid biomedical waste in common dumpsites. While many studies reported ... -
Application of magnetic cytosmear for the estimation of Plasmodium falciparum gametocyte density and detection of asexual stages in asymptomatic children
Sumari, Deborah; Grimberg, Brian T.; Blankenship, D’Arbra; Mugasa, Joseph; Mugittu, Kefas; Moore, Lee; Gwakisa, Paul; Zborowski, Maciej (Malaria Journal, 2016-02-24)Background: Conventional malaria parasite detection methods, such as rapid diagnostic tests (RDT) and light microscopy (LM), are not sensitive enough to detect low level parasites and identification of gametocytes in ... -
Assessing Risk Factors for Trypanosome Infections in Cattle in Wildlife Interface Areas in Northern Tanzania
Ngongolo, Kelvin; Estes, Anna; Hudson, Peter; Gwakisa, Paul (Journal of Infectious Diseases and Epidemiology, 2019-05-04)Trypanosomosis is a vector-borne, tropical disease that causes mortality and morbidity in livestock and humans. In this study we investigated the risk factors for trypanosome infection in cattle in the Maasai Steppe of ... -
CD5+ B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense-infected cattle
Buza, Joram; Sileghem, Maarten; Gwakisa, Paul; Naessens, Jan (Blackwell Science Ltd, 1997)Mice infected with African trypanosomes produce exceptionally large amounts of serum IgM, a major part of which binds to non-trypanosome antigens such as trinitrophenol and single-strand DNA. In this paper, we describe ... -
Detection of carrier state and genetic diversity of Theileria parva in ECF-vaccinated and naturally exposed cattle in Tanzania
Magulu, Emelesiana Cyprian; Kindoro, Fatuma; Mwega, Elisa; Kimera, Sharadhuli; Shirima, Gabriel; Gwakisa, Paul (Elsevier B.V., 2019-08)Infection and Treatment Method (ITM) has been practiced in Tanzania for over 20 years as a prevention measure against East Coast Fever disease. It is known that ITM, like natural ECF infection, leads to a carrier state, ... -
Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
Katani, Robab; Schilling, Megan; Lyimo, Beatus; Eblate, Ernest; Martin, Andimile; Tonui, Triza; Cattadori, Isabella; Francesconi, Stephen; Estes, Anna; Rentsch, Dennis; Srinivasan, Sreenidhi; Lyimo, Samson; Munuo, Lidia; Tiambo, Christian; Stomeo, Francesca; Gwakisa, Paul; Mosha, Fausta; Hudson, Peter; Buza, Joram; Kapur, Vivek (Springer Nature Limited, 2021-07-21)Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in ... -
iMAP: an integrated bioinformatics and visualization pipeline for microbiome data analysis
Buza, Teresia; Tonui, Triza; Stomeo, Francesca; Tiambo, Christian; Katani, Robab; Schilling, Megan; Lyimo, Beatus; Gwakisa, Paul; Cattadori, Isabella; Buza, Joram; Kapur, Vivek (BioMed Central, 2019-07-03)One of the major challenges facing investigators in the microbiome field is turning large numbers of reads generated by next-generation sequencing (NGS) platforms into biological knowledge. Effective analytical workflows ... -
iMAP: an integrated bioinformatics and visualization pipeline for microbiome data analysis
Buza, Teresia; Tonui, Triza; Stomeo, Francesca; Tiambo, Christian; Katani, Robab; Schilling, Megan; Lyimo, Beatus; Gwakisa, Paul; Cattadori, Isabella; Buza, Teresia; Kapur, Vivek (BMC Bioinformatics, 2019)Background: One of the major challenges facing investigators in the microbiome field is turning large numbers of reads generated by next-generation sequencing (NGS) platforms into biological knowledge. Effective analytical ... -
Influence of Land Cover and Host Species on Trypanosome Infection in Cattle and its Socio-Economic Impacts to Pastoralists of the Maasai Steppe, Tanzania
Ngongolo, Kelvin; Shirima, Gabriel; Mpolya, Emmanuel; Estes, Anna; Hudson, Peter; Gwakisa, Paul (Journal of Infectious Diseases and Epidemiology, 2020-01-27)Introduction Trypanosome infections result into trypanosomosis in cattle and this is an infection detrimental to pastoralist income. The patterns of transmission are thought to be influenced by ecological factors including ... -
Molecular species identification of bushmeat recovered from the Serengeti ecosystem in Tanzania.
Schilling, Megan; Estes, Anna; Eblate, Ernest; Martin, Andimile; Rentsch, Dennis; Katani, Robab; Joseph, Asteria; Kindoro, Fatuma; Lyimo, Beatus; Radzio-Basu, Jessica; Cattadori, Isabella; Hudson, Peter; Kapur, Vivek; Gwakisa, Paul; Buza, Joram (PLOS ONE, 2020-09-14)Bushmeat harvesting and consumption represents a potential risk for the spillover of endemic zoonotic pathogens, yet remains a common practice in many parts of the world. Given that the harvesting and selling of bushmeat ... -
Prevalence and Antimicrobial Resistance Phenotype of Enteric Bacteria from a Municipal Dumpsite
Mwaikono, Kilaza Samson; Maina, Solomon; Gwakisa, Paul (Science and Education Publishing, 2015)The objective of the study was to determine the prevalence and antibiotic resistance phenotype of enteric bacteria from the municipal dumpsite. A qualitative survey of the dumpsite was conducted to identify types of solid ...