• Login
    View Item 
    •   NM-AIST Home
    • Life sciences and Bio-engineering
    • Masters Theses and Dissertations [LiSBE]
    • View Item
    •   NM-AIST Home
    • Life sciences and Bio-engineering
    • Masters Theses and Dissertations [LiSBE]
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Formulation, Optimization, and Evaluation of Moringa oleifera leaf polyphenol-loaded phytosome delivery system against breast cancer cell lines

    Thumbnail
    View/Open
    Full text (12.78Mb)
    Date
    2022-07
    Author
    Ndung’u, Jecinta
    Metadata
    Show full item record
    Abstract
    Active plant molecules like polyphenols have been repositioned increasingly utilized for managing breast cancer traditionally. However, polyphenols possess poor lipid miscibility, that results in poor bioavailability and decreased efficacy. ‘Phytosome’ is a plant based nanocarrier that improves the bioavailability of the polyphenols. The purpose of this study was to improve the bioavailability of Moringa oleifera Lam leaf polyphenols (Mopp) through encapsulation with phytosomes to enhance their activity on 4T1 cancer cell lines. The Mopp phytosomes were extracted by microwave-assisted extraction. Moringa oleifera polyphenols-loaded phytosomes (MoP) were prepared by the nanoprecipitation method and characterized by a dynamic light scattering technique. The ultraviolet-visible spectroscopy determined the percentage entrapment efficiency (EE%). Fourier-transform infrared (FTIR) spectroscopy assessed MoP formation. The dialysis membrane technique investigated in vitro drug release while storage stability test analysis identified MoP stability. The in vitro cytotoxic activity of MoP on E6 Vero cell lines and anti-proliferative activity on 4T1 cancer cell lines were investigated by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, while female Swiss albino mice were used to assess acute toxicity. Results showed that MoP average particle size was 296±0.29 nm, a zeta-potential of -40.1±1.19 mV, and polydispersity index of 0.106±0.002. The Mopp total phenolic content was 50.81±0.02 mg GAE/g, EE% was 90.32±0.11%, while FTIR spectra demonstrated successful formation of phytosomes. The drug release profiles demonstrated biphasic behavior of immediate burst release and subsequent prolonged sustained release. The in vitro cytotoxicity assays indicated that the MoP had low cytotoxic effect of 98.84±0.53 μg/mL on E6 Vero cells; while doxorubicin had 68.35±3.51while Mopp was 212.9±1.30 μg/mL. Moreover, MoP exhibited the highest antiproliferative effect on 4T1 cancer cells with an inhibitory concentration of 7.73±2.87 μg/mL and selectivity index >3. The results indicated a significant difference (p≤ 0.001) in MoP when compared to Mopp and doxorubicin. In vivo investigation confirmed the safety of MoP on mice at a dose below 2000 mg/kg. The present findings suggest that the MoP can serve as an effective and promising formulation for breast cancer drug delivery in cancer therapy.
    URI
    https://doi.org/10.58694/20.500.12479/1498
    Collections
    • Masters Theses and Dissertations [LiSBE]

    Nelson Mandela-AIST copyright © 2021  DuraSpace
    Theme by 
    Atmire NV
     

     

    Browse

    All PublicationsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Nelson Mandela-AIST copyright © 2021  DuraSpace
    Theme by 
    Atmire NV