Curdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities

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dc.contributor.author Tukulula, Matshawandile
dc.contributor.author Hayeshi, Rose
dc.contributor.author Fonteh, Pascaline
dc.contributor.author Meyer, Debra
dc.contributor.author Ndamase, Abongile
dc.contributor.author Madziva, Michael T.
dc.contributor.author Khumalo, Vincent
dc.contributor.author Lubuschagne, Philip
dc.contributor.author Naicker, Brendon
dc.contributor.author Swai, Hulda
dc.contributor.author Dube, Admire
dc.date.accessioned 2019-10-18T07:52:58Z
dc.date.available 2019-10-18T07:52:58Z
dc.date.issued 2015-03-28
dc.identifier.uri DOI 10.1007/s11095-015-1655-9
dc.identifier.uri http://dspace.nm-aist.ac.tz/handle/123456789/504
dc.description Research Article published by Springer en_US
dc.description.abstract Purpose There is significant interest in the application of nanoparticles to deliver immunostimulatory signals to cells.We hypothesized that curdlan (immune stimulating polymer) could be conjugated to PLGA and nanoparticles from this copolymer would possess immunostimulatory activity, be non-cytotoxic and function as an effective sustained drug release system. Methods Carbodiimide chemistry was employed to conjugate curdlan to PLGA. The conjugate (C-PLGA) was characterized using 1H and 13C NMR, FTIR, DSC and TGA. Nanoparticles were synthesized using an emulsion-solvent evaporation technique. Immunostimulatory activity was characterized in THP-1 derived macrophages. MTTassay and real-time impedance measurements were used to characterize polymer and nanoparticle toxicity and uptake in macrophages. Drug delivery capability was assessed across Caco-2 cells using rifampicin as a model drug. Results Spectral characterization confirmed successful synthesis of C-PLGA. C-PLGA nanoparticles enhanced phosphorylated ERK production in macrophages indicating cell stimulation. Nanoparticles provided slow release of rifampicin across Caco-2 cells. Polymers but not nanoparticles altered the adhesion profiles of the macrophages. Impedance measurements suggested Ca2+ dependent uptake of nanoparticles by the macrophages. Conclusions PLGA nanoparticles with macrophage stimulating and sustained drug delivery capabilities have been prepared. These nanoparticles can be used to stimulate macrophages and concurrently deliver drug in infectious disease therapy. en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.subject immunostimulantnanoparticles en_US
dc.subject real-time impedance measurements en_US
dc.subject PLGA nanoparticles en_US
dc.title Curdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities en_US
dc.type Article en_US

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