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Comparative in vitro transportation of pentamidine across the blood-brain barrier using polycaprolactone nanoparticles and phosphatidylcholine liposomes

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dc.contributor.author Omarch, Geofrey
dc.contributor.author Kippie, Yunus
dc.contributor.author Mentor, Shireen
dc.contributor.author Ebrahim, Naushaad
dc.contributor.author Fisher, David
dc.contributor.author Murilla, Grace
dc.contributor.author Swai, Hulda
dc.contributor.author Dube, Admire
dc.date.accessioned 2019-10-18T07:42:13Z
dc.date.available 2019-10-18T07:42:13Z
dc.date.issued 2019-04-22
dc.identifier.uri https://doi.org/10.1080/21691401.2019.1596923
dc.identifier.uri http://dspace.nm-aist.ac.tz/handle/123456789/503
dc.description Research Article published by Taylor & Francis Group VOL. 47, NO. 1, 2019 en_US
dc.description.abstract Nanoparticles (NPs) have gained importance in addressing drug delivery challenges across biological barriers. Here, we reformulated pentamidine, a drug used to treat Human African Trypanosomiasis (HAT) in polymer based nanoparticles and liposomes and compared their capability to enhance pentamidine penetration across blood brain barrier (BBB). Size, polydispersity index, zeta potential, morphology, pentamidine loading and drug release profiles were determined by various methods. Cytotoxicity was tested against the immortalized mouse brain endothelioma cells over 96 h. Moreover, cells monolayer integrity and transportation ability were examined for 24 h. Pentamidine-loaded polycaprolactone (PCL) nanoparticles had a mean size of 267.58, PDI of 0.25 and zeta potential of –28.1 mV and pentamidine-loaded liposomes had a mean size of 119.61 nm, PDI of 0.25 and zeta potential 11.78. Pentamidine loading was 0.16 mg/mg (w/w) and 0.17 mg/mg (w/w) in PCL NPs and liposomes respectively. PCL nanoparticles and liposomes released 12.13% and 22.21% of pentamidine respectively after 24 h. Liposomes transported 87% of the dose, PCL NPs 66% of the dose and free pentamidine penetration was 63% of the dose. These results suggest that liposomes are comparatively promising nanocarriers for transportation of pentamidine across BBB. en_US
dc.language.iso en en_US
dc.publisher Taylor & Francis Group. en_US
dc.subject transendothelial electrical resistance en_US
dc.subject Human African Trypanosomiasis en_US
dc.subject blood brain barrier en_US
dc.title Comparative in vitro transportation of pentamidine across the blood-brain barrier using polycaprolactone nanoparticles and phosphatidylcholine liposomes en_US
dc.type Article en_US


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