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Polymorphisms of the Chicken Mx Gene Promoter and Association with Chicken Embryos’ Susceptibility to Virulent Newcastle Disease Virus Challenge

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dc.contributor.author Mpenda, Fulgence N.
dc.contributor.author Keambou, Christian T.
dc.contributor.author Kyallo, Martina
dc.contributor.author Pelle, Roger
dc.contributor.author Lyantagaye, Sylvester L.
dc.contributor.author Buza, Joram
dc.date.accessioned 2019-10-10T12:26:37Z
dc.date.available 2019-10-10T12:26:37Z
dc.date.issued 2019-10-03
dc.identifier.uri https://doi.org/10.1155/2019/1486072
dc.identifier.uri http://dspace.nm-aist.ac.tz/handle/123456789/483
dc.description Research Article published by Hindawi en_US
dc.description.abstract Newcastle disease is a devastating viral disease of chicken in low- and middle-income countries where the backyard production system is predominant. Marker-assisted selection of chickens that are resistant to Newcastle disease virus (NDV) is the promising strategy that needs to be explored. )e aim of the present study was to investigate polymorphisms of the promoter region of the chicken Mx gene and association with Kuroiler, Sasso, and local Tanzanian chicken embryos’ survival variability to virulent NDV infection. Chicken embryos were initially challenged with a minimum lethal dose of virulent NDV suspension and then were followed over time to gather information on their survival variability. Using the survival data, high and less susceptible cohorts were established, and a total of 88 DNA samples from high and less susceptible groups were genotypes by sequencing. Five singlenucleotide polymorphisms (SNPs), which were previously reported, were detected. Interestingly, for the first time, the findings demonstrated the association of the promoter region of chicken myxovirus-resistance (Mx) gene polymorphisms with chicken embryos’ susceptibility to the virulent NDV challenge. At the genotypic level, the SNP4 G>A mutation that was located within the IFN-stimulating response element was associated (LR: 6.97, P � 0.03) with chicken embryos’ susceptibility to the virulent NDV challenge. An allele G frequency was higher in the less susceptible cohort, whereas an allele A frequency was higher in the high susceptible cohort. At the haplotype level, the haplotype group ACGC was associated (OR: 9.8, 95% CI: 1.06–79.43, P � 0.042) with the same trait and had a resistant effect. In conclusion, the results have demonstrated the association of chicken Mx gene promoter polymorphisms and chicken embryos’ survival variability to the virulent NDV challenge, and the information is useful for breeding programs designed to develop chicken genotypes that are resistant to Newcastle disease virus. en_US
dc.language.iso en en_US
dc.publisher Hindawi en_US
dc.subject Research Subject Categories::NATURAL SCIENCES en_US
dc.title Polymorphisms of the Chicken Mx Gene Promoter and Association with Chicken Embryos’ Susceptibility to Virulent Newcastle Disease Virus Challenge en_US
dc.type Article en_US


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