Ribosomal/nucleolar stress induction regulates tert-Butyl hydroperoxide (tBHP) mediated oxidative stress in Anopheles gambiae midguts.

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dc.contributor.author Tarimo, Brian B
dc.contributor.author Hritzo, Bernadette A
dc.contributor.author Law, Henry Chun Hin
dc.contributor.author Tao, Dingyin
dc.contributor.author Pastrana-Mena, Rebecca
dc.contributor.author Kanzok, Stefan M
dc.contributor.author Buza, Joram J.
dc.contributor.author Dinglasan, Rhoel R
dc.date.accessioned 2019-07-30T11:07:36Z
dc.date.available 2019-07-30T11:07:36Z
dc.date.issued 2019-03-29
dc.identifier.issn 1756-0500
dc.identifier.other 30922378
dc.identifier.uri https://doi.org/10.1186/s13104-019-4196-1
dc.identifier.uri http://dspace.nm-aist.ac.tz/handle/123456789/391
dc.description Research Article published by BMC Research Notes en_US
dc.description.abstract Objective: A fundamental understanding of redox homeostasis in Anopheles gambiae midgut cells under different oxidative conditions is missing. Such knowledge can aid in the development of new malaria transmission-blocking strategies aimed at disrupting natural homeostatic processes in the mosquito during Plasmodium parasite uptake (i.e. blood feeding). The aim of this study was to understand how the An. gambiae midgut regulates oxidative stress to reactive oxygen species (ROS), especially to a potent ROS-inducer such as tert-Butyl hydroperoxide (tBHP). Results: Initial studies using quantitative immunoblot indicated that the expression of the classical antioxidant protein An. gambiae thioredoxin-1 (AgTrx-1) remained unchanged across challenges with different concentrations of tBHP suggesting that additional mechanisms to regulate ROS may be involved. We therefore conducted a global proteomic survey, which revealed that An. gambiae midguts under low (50 μM) and high (200 μM) tBHP concentrations were enriched in proteins indicative of ribosomal/nucleolar stress. Ribosomal stress is an inherent cellular response to an imbalance in ribosomal proteins (RPs) due to cellular stress such as oxidative stress. Our data suggest that ribosomal/ nucleolar stress is the primary cellular response in An. gambiae midguts under tBHP challenge. Considering these results, we discuss harnessing the ribosomal stress response as a potential malaria transmission-blocking strategy. en_US
dc.language.iso en en_US
dc.publisher BMC Research Notes en_US
dc.subject Anopheles gambiae en_US
dc.subject Malaria en_US
dc.subject Nucleolar stress en_US
dc.subject Oxidative stress en_US
dc.subject Plasmodium en_US
dc.subject Ribosomal stress en_US
dc.subject Thioredoxin en_US
dc.subject Transmission-blocking en_US
dc.subject tert-Butyl hydroperoxide en_US
dc.title Ribosomal/nucleolar stress induction regulates tert-Butyl hydroperoxide (tBHP) mediated oxidative stress in Anopheles gambiae midguts. en_US
dc.type Article en_US

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