xmlui.dri2xhtml.structural.head-subtitle

Application of magnetic cytosmear for the estimation of Plasmodium falciparum gametocyte density and detection of asexual stages in asymptomatic children

Show simple item record

dc.contributor.author Sumari, Deborah
dc.contributor.author Grimberg, Brian T.
dc.contributor.author Blankenship, D’Arbra
dc.contributor.author Mugasa, Joseph
dc.contributor.author Mugittu, Kefas
dc.contributor.author Moore, Lee
dc.contributor.author Gwakisa, Paul
dc.contributor.author Zborowski, Maciej
dc.date.accessioned 2019-05-24T08:27:53Z
dc.date.available 2019-05-24T08:27:53Z
dc.date.issued 2016-02-24
dc.identifier.uri DOI 10.1186/s12936-016-1170-4
dc.identifier.uri http://dspace.nm-aist.ac.tz/handle/123456789/196
dc.description Research Article published by Malaria Journal en_US
dc.description.abstract Background: Conventional malaria parasite detection methods, such as rapid diagnostic tests (RDT) and light microscopy (LM), are not sensitive enough to detect low level parasites and identification of gametocytes in the peripheral blood. A modified and sensitive laboratory prototype, Magnetic Deposition Microscopy (MDM) was developed to increase the detection of sub-microscopic parasitaemia and estimation of gametocytes density in asymptomatic school children. Methods: Blood samples were collected from 303 asymptomatic school children from seven villages in Bagamoyo district in Tanzania. Participants were screened for presence of malaria parasites in the field using RDT and MDM whereas further examination of malaria parasites was done in the laboratory by LM. LM and MDM readings were used to calculate densities and estimate prevalence of asexual and sexual stages of the parasite. Results: Plasmodium falciparum parasites (asexual and sexual stages) were detected in 23 (7.6 %), 52 (17.2 %), and 59 (19.5 %) out of 303 samples by LM, RDT and MDM respectively. Gametocytes were detected in 4 (1.3 %) and 12 (4.0 %) out of the same numbers of samples by LM, and MDM, respectively. Likewise, in vitro results conducted on two laboratory strains of P. falciparum, 3D7 and NF54 to assess MDM sensitivity on gametocytes detection and its application on concentrating gametocytes indicated that gametocytes were enriched by MDM by 10-fold higher than LM. Late stages of the parasite strains, 3D7 and NF54 were enriched by MDM by a factor of 20.5 and 35.6, respectively. MDM was more specific than LM and RDT by 87.5 % (95 %, CI 71.2–89.6 %) and 89.0 % (95 % CI 82.9–91.4) respectively. It was also found that MDM sensitivity was 62.5 % (95 % CI 49.5–71.8) when compared with RDT while with LM was 36.5 % (95 % CI 32.2–60.5). Conclusions: These findings provide strong evidence that MDM enhanced detection of sub-microscopic P. falciparum infections and estimation of gametocyte density compared to current malaria diagnostic tools. In addition, MDM is superior to LM in detecting sub-microscopic gametocytaemia. Therefore, MDM is a potential tool for low-level parasitaemia identification and quantification with possible application in malaria transmission research. en_US
dc.language.iso en_US en_US
dc.publisher Malaria Journal en_US
dc.subject Asymptomatic malaria en_US
dc.title Application of magnetic cytosmear for the estimation of Plasmodium falciparum gametocyte density and detection of asexual stages in asymptomatic children en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search Our NM-AIST IR


Browse NM-AIST IR

My NM-AIST IR Account

Statistics